摘要
目的:建立刺玫果中金丝桃苷含量测定的方法。方法:采用高效液相色谱法测定,色谱柱为Shim-pack VP-ODS C18(4.6mm×150mm,5μm);以甲醇(A)和0.2%磷酸溶液(B)进行梯度洗脱,流速为1.0mL/min;进样量20μL;检测波长360nm;柱温40℃。结果:金丝桃苷进样质量浓度在4.76～76.16μg/mL范围内呈良好的线性关系,r=0.9993。平均回收率为98.06%,RSD为1.72%。结论:采用高效液相色谱法测定刺玫果中金丝桃苷的含量,方法简便、准确,可以用于刺玫果的质量控制。
Objective： To establish a determination method for hyperin in Rosa davurica Pall.. Methods： A HPLC method was developed using Shim-pack VP-ODS C18 （4.6 mm × 150 mm, 5 μm） column as the stationary phase and CH3OH-0.2% H3PO4 as the mobile phase. The flow rate of mobile phase, injection volume of the sample and the column temperature were 1.0 mL/min, 20 μL and 40 ℃, respectively. The UV detection wavelength was 360 nm. Results： A good linear relationship between integral value of peak area and hyperin concentration in the range of 4.76-76.16 μg/mL （r = 0.9993） was observed. The average recovery rate of hyperin was 98.06% with the RSD of 1.72%. Conclusion： The HPLC determination of hyperin in Rosa davurica Pall. was characteristics of convenience and accuracy, which can be used for the quality control of Rosa davurica Pall..
出处
《食品科学》
EI
CAS
CSCD
北大核心
2010年第24期281-284,共4页
Food Science
基金
吉林省教育厅重点计划项目(吉教科合字2006第122号)
