摘要
以新椒系列辣椒杂交种父母本和一代杂种为材料,采用液氮-SDS法提取辣椒基因组DNA,研究PCR反应的主要影响因子,建立适合辣椒杂交种纯度RAPD分析的PCR反应体系,从120个随机引物中筛选出1个能鉴定新椒10号杂种纯度的引物S1220,从7个随机引物中筛选出1个能鉴定新椒3号杂种纯度的引物S1213。
Some F1 hybrids in Xinjiao hot pepper series and their parents were used as experimental materials in this study.The genomic DNA was extracted with liquid N2-SDS method and the PCR system that was suitable for RAPD analysis of these materials was established.The results showed that primer S1220 could test the purity of F1 hybrid A effectively and S1213 could test the purity of F1 hybrid B effectively.
出处
《辣椒杂志》
2010年第4期29-32,共4页
Journal of China Capsicum
关键词
辣椒
杂交种
RAPD分析
纯度鉴定
hot pepper
hybrid
RAPD analysis
purity identification
