摘要
为了研究临床分离多重耐药大肠杆菌的耐药机制，我们采用ＰＣＲ和Ｓｏｕｔｈｅｒｎｂｌｏｔ检测大肠杆菌主动外排系统ＡｃｒＡＢ结构基因ａｃｒＡＢ在临床分离菌株中的分布并用ＲＴ－ＰＣＲ测定其ｍＲＮＡ表达水平。结果在所有的临床分离大肠杆菌染色体中均发现有ａｃｒＡＢ基因，未发现ａｃｒＡＢ缺失株；大肠杆菌多重耐药株ａｃｒＡＢ的ｍＲＮＡ水平明显高于敏感野生株和其它耐药谱不同的菌株，而敏感野生株和耐药谱不同的菌株间无差别。本研究表明大肠杆菌ａｃｒＡＢ表达水平决定了大肠杆菌多重耐药表型，ｍａｒ可能调控ａｃｒＡＢ的表达。
To investigate multiple-antibiotic-resistance mechanism in Escherichia coli, the distribution of active efflux pump gene, acrAB, were determined by PCR, Southern bolt, and its expressions by RT-PCR, in clinical strains of Escherichia coli. The results showed acrAB gene was found in all clinical isolates of Escherichia coli, and was not found in acrAB-deficient strain, and the levels of expression of acrAB in multiple_antibiotic_resistance (Mar) strains were significantly higher than that in wild susceptible_ and others strains. The investigation suggests that Mar phenotype of Escherichia coli be determined by the levels of active efflux system acrAB gene expression, and the expression of acrAB gene should be regulated by mar operon.
出处
《中国临床药理学杂志》
CAS
CSCD
北大核心
1999年第3期171-174,共4页
The Chinese Journal of Clinical Pharmacology
基金
中国博士后科学基金
