摘要
采用改进的CAS定性、定量方法,从16株PGPR菌株中初步筛选出具有抗病原真菌作用的菌株,再利用平板对峙法将筛选出的5株PGPR菌与3种病原真菌进行拮抗试验。结果表明,LHS11对黄瓜枯萎病菌(Fusarium oxysporum f.sp.cucumerinum),西瓜枯萎病菌(Fusarium oxysporum f.sp.niveum)的抑制效果最好,抑菌率达到80%以上;其次是191对黄瓜枯萎病菌,抑菌率为74%,LHS11、191对立枯丝核菌(Rhizoctonia solani)的抑制效果相近,抑菌率分别为64%和65%。191和LHS11是抑菌效果较好的生防PGPR菌株,具有较好的应用潜力。
By adopting improved CAS methods, the strains antagonistic against plant pathogens were firstly screened from the 16 PGPR strains, and the antagonistic experiments were then conducted by using 5 PGPR strains to 3 types of plant pathogens using plate cultivation. The results showed that LHSll had the best inhibitory effects on Fusarium oxysporum f. sp. cucurnerinum and F. oxysporum f. sp. niveum, with the inhibition ratio over 80% ; the inhibition ratio of 191 against F. oxysporum f. sp. cucumerinum was 74%. The inhibitory effects of LHS11 and 191 on Rhizoctonia solani were similar （ 64% and 65%, respectively）. Both 191 and LHSll could be used as PGPR biocontrol agents with good inhibitory effects and high application potential.
出处
《植物保护》
CAS
CSCD
北大核心
2011年第1期59-64,共6页
Plant Protection
基金
国家科技支撑计划基金(2007BAD89B17)
国家自然科学基金(30960265)
农业部燕麦产业体系基金
贵州省科技攻关项目(黔科合NY字[2009]3067)
