摘要
采用Southernblot和狭缝（Slotblot）杂交的方法，对高效表达人尿激酶原（pro-UK）的工程细胞──11G原代细胞及传134代后细胞含有的pro-UK基因拷贝数进行了测定。结果显示，11G工程细胞株内所含的pro-UK的拷贝数为100—200拷贝／细胞，传134代后其拷贝数基本不变，说明11G细胞株是稳定高表达pro-UK的工程细胞，符合WHO规定的关于重组DNA转化细胞用于生产的要求。
Southern blot and Slot blot hybridization methods were used in this research to detect the copy numbers of pro-urokinase (pro-UK) gene in pro-UK expression engineering cell line (11G cell line and prostated 134 times cell line). The results showed that the copy ntmbers of pro-UK gene were about 100-200 copies/cell and the copy numbers did not changed after propapahfing 134 bines. This demonstrated that pro-UK gene expression can be stable and high efficient in 11G engineering cell line and it is suitable for WHO requeshon about recombinant DNA transfected cell line for preducts.
出处
《微生物学通报》
CAS
CSCD
北大核心
1999年第3期162-165,共4页
Microbiology China
关键词
尿激酶原
拷贝数
工程细胞株
杂交
Pro-urokinase
Copy numbed
Engineering cell line
Hybridization
