摘要
目的比较3种不同化学修饰的水分散性碳纳米管[磷酰胆碱(phosphoryl choline,PC)修饰碳纳米管(CNT-PC)、聚乙二醇修饰碳纳米管(CNT-PEG)、磷酰胆碱和聚乙二醇修饰碳纳米管(CNT-PEG-PC)]在大鼠体内的急性毒性、分布和其他生物效应。方法将144只清洁级SD大鼠按体重随机分为8组,分别为对照(PBS缓冲溶液)组和12.5、25、50、70、100、140、200 mg/kg CNTs染毒组,每组6只,雌雄各半。采用尾静脉注射方法进行1次性染毒,注射容量为10 ml/kg。观察动物反应及死亡情况。染毒7 d后,检测全血淋巴细胞数量和血清白蛋白(ALB)、丙氨酸氨基转移酶(ALT)、肌酐(Cr)含量,并进行组织病理学观查。结果当3种碳纳米管修饰物的注射剂量>25 mg/kg时,与有PC基团修饰的CNTs相比,CNT-PEG更容易导致大鼠的窒息死亡;含有PEG基团结构的CNTs在大鼠血液循环系统中滞留时间更长,而CNT-PC则更容易进入肺、肝和脾组织中,并且分散良好;各剂量CNT-PC、CNT-PEG和CNT-PEG-PC染毒组大鼠血清ALB、ALT和Cr含量间比较,差异无统计学意义。CNT-PC染毒大鼠血液淋巴细胞数量随注射剂量的升高而逐渐增加;且与对照组比较,50～200 mg/kg的CNT-PC染毒组大鼠血液淋巴细胞数量较多,差异均有统计学意义(P<0.05)。经3种修饰过的CNTs染毒后,在大鼠体内均未发现炎症和病变。结论 3种CNTs修饰物都没有表现出明显的急性毒性;但由于修饰基团不同化学结构的作用,其在大鼠体内表现出不同的分布和生物效应。
Objective To evaluate the acute toxicity,the bio-distribution and the other biological effects of three types of water-soluble carbon nanotube derivates. Methods One hundred and forty-four Sprague-Dawley （SD） rats were randomly divided into seven dose groups and a control group and treated with CNTs throughh caudal vein injection at the dosages of 12.5,25,50,70,100,140 and 200 mg/kg,6 rats each group. The injection volume was 10 ml/mg. One week later,rats were sacrificed by exsanguinations＇ via the femoral aorta, Whole blood as well as the liver, spleen, lung tissues were collected for further assay. Results At extremely high doses,CNT-PEG was easier to cause the death of rats than CNT-PC and CNT-PEG-PC. Compared with CNT-PEG and CNT-PEG-PC, CNT-PC was obviously easier to penetrate into lung tissues and ingested by liver and spleen tissues. The biochemical indices such as ALB, ALT and Cr did not have significant changes compared with the control groups （P〉0.05）. CNT-PC could induce the proliferation and activation of lymphocytes （P〈0.05）. There was no obvious clinical manifestation of liver, spleen or lung injury in rats after 7-day exposure of all of three types of CNT derivates. Conclusion Three kinds of modified CNTs did not show obvious acute toxicity to the rats, but the CNTs with different chemical constitution may induce distinct behaviors of CNTs in vivo such as bio-distribution and other biological effects.
出处
《环境与健康杂志》
CAS
CSCD
北大核心
2011年第1期6-10,F0003,共6页
Journal of Environment and Health
基金
国家自然科学基金(20874043)
国家重点基础研究发展计划("973")项目(2006CB705602x)
关键词
碳纳米管
化学修饰
体内毒性
Carbon nanotubes
Chemical modification
In-vivo toxicity
