摘要
利用傅里叶变换红外光谱(FTIR)分析食源性致病菌脂多糖粗提物,建立一种微生物快速筛选、鉴别的方法。分析5种(8株)典型革兰氏阴性食源性致病菌脂多糖的红外光谱特征区段的差异,采用分级聚类分析方法对结果进行比较,并用菌株16s rDNA的亲缘性分析进行比对验证。结果表明,革兰氏阴性食源性致病菌脂多糖的光谱特征区间分别为1750～1500cm-1、1500～1360cm-1、1160～920cm-1和890～750cm-1。聚类结果显示,基于脂多糖的红外光谱分析方法,不仅可以区分不同种属的微生物、致病与非致病性食源性致病菌,而且还能将同种不同分离株的食源性致病菌归类聚合。FTIR结合多元分析方法可用于食源性致病菌的快速筛选研究。
A rapid identification methods of microbial species was established by the Fourier transform infrared（FTIR） spectra of crude lipopolysaccharides extracted from five different kinds of gram-negative food-borne bacteria.According to the difference in each strain＇s characteristic range,their relationship were compared and identified by clustering method.In addition,the method had been proved to be reliable by the analysis of clustering and phylogenetic tree based on 16srDNA.The result showed that the characteristic ranges of gram-negative food-borne bacterial were 1 750～1 500 cm-1,1 500～1 360 cm-1,1 160～920 cm-1 and 890～750 cm-1.Different species of food-borne pathogens can be identified significantly based on the method of FTIR to crude lipopolysaccharides.With these protocols,virulent and avirulent food-borne pathogens can be identified.The result of clustering also demonstrated that this method can classify the strains which were separated from a same species.FTIR combined with a multidimensional approach show high potential as a rapid,accurate and easy-to-use method for the identification of food-borne pathogens.It will be an important practical tool in the identification of food-borne pathogens studies.
出处
《中国食品学报》
EI
CAS
CSCD
北大核心
2011年第1期202-207,共6页
Journal of Chinese Institute Of Food Science and Technology
基金
上海市自然科学基金(10ZR1421900)
上海市科委长三角项目(10595811900)
关键词
傅里叶变换红外光谱(FTIR)
脂多糖
鉴别
聚类分析(HCA)
fourier transforms infrared spectroscopy（FTIR）
lipopolysaccharides（LPS）
identification
hierarchical cluster analysis（HCA）
