摘要
目的:用正交实验提取吴茱萸中有效成分吴莱萸碱,优选吴茱萸的提取路线。方法:采用高效液相色谱(HPLC)法测定吴茱萸酸水提物中吴茱萸碱的含量,求出最佳条件。结果:实验表明,酸水浓度、浸泡时间对吴茱萸碱的提取影响较大,煎煮时间则次之。结论:经筛选,吴莱萸中生物碱水提取的工艺路线为:吴茱萸浸泡1.5 h、煎煮30 min、醋酸浓度为1%,在此条件下吴茱萸中生物碱提取完全。
Objective：To Extract rutaecarpine which is the effective component in Evodia rutaecarpa by orthogonal experiment and then optimize the extracting course.Methods：To determine the content of rutaecarpine which was extracted from the aqueous extract of xanthoxylic acid and work out the optimum condition through the way of HPLC.Results：The experiment showed that decoction time and menstruum dosage had more significant influence on extracting rutaecarpine,and decoction hour took the second place.Conclusion：Through the comparison,the best technique to extract rutaecarpine from Evodia rutaecarpa is marinating Evodia rutaecarpa for 1.5 h,then another half hour for decoction,with the 1% consistency of acetic acid.Under this condition,the rutaecarpine is extracted completely.
出处
《中医学报》
CAS
2011年第2期190-191,共2页
Acta Chinese Medicine
关键词
吴茱萸碱
中药提取工艺
正交试验法
Evodia rutaecarpm
extracting processing of chinese herbs
orthogonal design
