摘要
目的:研究知柏地黄汤对解脲支原体(Uu)感染大鼠睾丸组织IL-2、TNF-α表达水平的影响,探讨该方防治Uu感染所致的男性不育症的部分免疫学机制。方法:经膀胱接种建立大鼠睾丸组织感染解脲支原体的动物模型。实验设知柏地黄汤治疗组、美满霉素治疗组、模型对照组和假手术组。假手术组、模型对照组灌胃给生理盐水,治疗组灌胃给相应药物,连续给药21d后取标本检测指标。免疫组织化学染色法和Western blotting技术检测睾丸组织IL-2、TNF-α蛋白表达水平,RT-PCR技术检测睾丸组织IL-2、TNF-α基因表达水平。结果:Uu感染大鼠后,睾丸组织IL-2的蛋白表达和基因表达水平降低,TNF-α的蛋白表达和基因表达水平升高,与假手术组比较,差异显著(P<0.01);知柏地黄汤组睾丸组织IL-2蛋白表达和基因表达水平明显升高,TNF-α的蛋白表达和基因表达水平明显降低,与模型对照组比较,差异显著(P<0.01)。结论:知柏地黄汤能通过影响Uu感染大鼠睾丸组织IL-2、TNF-α的表达水平而发挥抗感染作用。
Objective： In order to know the immune mechanism of Zhibai Dihuang Decoction（ZHBDHD） in treating male infertility by ureaplasma urealyticum（Uu）,we performed the research for the effects of ZHBDHD on the expression of IL-2 TNF-α in the rats infected by Uu.Methods： Rats model was prepared by inoculating Uu in the bladder of rats.These rats were randomly divided into four groups： ZHBDHD group,minocycline group,model group and false operation group.The rats of model group and false operation group were taken NS by garage,twice a day for continuing 21 days;ZHBDHD group were taken ZHBDHD and minocycline group taken minocycline by garage as the other two groups.Stop water and food for 8 hours after the last garage,taken samples for determining the contents of IL-2,TNF-α in testis by immunohistochemistry and western bloting;determining mRNA of IL-2,TNF-αin testis with RT-PCR.Result： Compared with false operation group,the expressions of IL-2 in testis were obviously decreased after infected by Uu.However,TNF-α in testis were found obviously increasing after rats infected by Uu comparing with false operation rats;On the contrary,ZHBDHD could increase the expression of IL-2,inhibit the expression of TNF-α.The results were same when ZHBDHD group was compared with minocycline group.Conclusion： ZHBDHD can exert anti-inflammatory by effecting the expression of IL-2 and TNF-α in the rats infected by Uu.
出处
《中华中医药杂志》
CAS
CSCD
北大核心
2011年第3期448-450,共3页
China Journal of Traditional Chinese Medicine and Pharmacy
基金
国家自然基金课题(No.30973759)
湖南省自然基金课题(No.09JJ3054)~~
关键词
知柏地黄汤
解脲支原体
白细胞介素-2
肿瘤坏死因子-α
Zhibai Dihuang Decoction（ZHBDHD）
Ureaplasma urealyticum
Interleukin-2
Tumor necrosis factor-α
