摘要
目的：建立HPLC-MS测定犬血浆中苯环喹溴铵浓度的方法。方法：色谱柱为Phenomcnex C18（2）100A（Luna 3μm，100mm×2．0mm），预柱为Luna C18（2）（3μm，10mm×2．0mm）柱，流动相为含1％甲酸的醋酸铵（8mmol·L^-1）溶液-甲醇（47：53），流速为0．20mL·min^-1；ESI选择性正离子检测。结果：在5～3600μg·L^-1范围内．苯环喹溴铵血药浓度呈线性关系，r=0．9994。最低检测限为3μg·L^-1，日内、日间精密度RSD均小于9．3％。结论：本方法专属性强，灵敏度高，准确性好，适合狗血浆中苯环喹溴铵的药动学研究。
OBJECTIVE HPLC-MS method was developed to determine the plasma concentration of bencydoquidium bromide in Beagle dogs. METHODS Chromatographic separation was achieved on a Phenomenex C18（2） 100A（Luna 3μm,100mm×2.0mm） column. Pre-column was Luna C18 （2） （3μm, 10 mm×2.0mm）. The mobile phase contained ammonium acetate with 1% formic acid and methanol 47：53（v/v）at a flow rate of 0. 20 mL min^- 1. HPLC-MS was carried out on a single quadrupole mass spectrometer using electrospray ionization（ESI） and positive selected ion monitoring （SMI）. RESULTS The linear range was 5 3600 μg·L^-1 for BCQB and the limit of detection（LOD） was 5 μg·L^-1. The intra-and inter-precisions （RSD） were all lower than 9. 3%. CONCLUSION The method is specific, sensitive,accurate and suitable for the pharmacokinetic study of BCQB in dogs.
出处
《中国医院药学杂志》
CAS
CSCD
北大核心
2011年第6期461-464,共4页
Chinese Journal of Hospital Pharmacy
