摘要
目的:探讨累加电针治疗神经源性痛大鼠的作用机制。方法:Wistar大鼠随机分为正常对照组,慢性压迫性损伤(CCI)组,CCI+EA2(天)D组,CCI+EA2(周)W组,去卵巢(OVX)+CCI组,OVX+CCI+EA2D组,OVX+CCI+EA2W组。去卵巢大鼠在手术后每天颈部皮下注射D-半乳糖,连续45天造成神经记忆损伤。结扎坐骨神经造成慢性神经疼痛模型,电针双侧"足三里"-"阳陵泉"穴1次/D,分别电针2D、2W。用RT-PCR的方法检测下丘脑前阿黑皮素(POMC)mRNA和海马、下丘脑孤啡肽前体(PPPNOC)mRNA的表达。结果:与正常对照组比,CCI组下丘脑POMC mR-NA表达水平显著升高(P<0.05),海马及下丘脑PPNOC mRNA表达水平也显著升高(P<0.05)。与CCI组比,CCI+EA2W组POMC mRNA表达水平进一步显著上调(P<0.05),而PPNOC mRNA表达水平显著降低(P<0.05)。在OVX+CCI动物上,与OVX+CCI组比,OVX+CCI+EA2W组下丘脑POMC的表达量明显上调(P<0.05),而OVX+CCI+EA2D和OVX+CCI+EA2W海马PPPNOC mRNA的表达显著下调(P<0.05),但后两组下丘脑PPPNOC mRNA的表达无显著变化(P>0.05)。结论:电针对慢性神经痛大鼠的累积性镇痛效应与下丘脑POMC mRNA表达的上调和海马PONC mRNA表达的下调密切相关;记忆损伤可减弱电针上调下丘脑POMC基因的表达。
Objective：To investigate the mechanism of repeated electroacupuncture （EA） underlying the cumulative analgesic effect in neuropathie pain rats. Methods ： A total of 35 Wistar rats were randomized into control （ n = 5 ） , chronic constrictive injury （CCI） （n=5）,CCI＋EA2day （D） （n=5）,ovaficctomy （OVX） ＋CCI （n=5）,OVX＋CCI＋EA2D （n=5）,OVX＋CCI＋ EA2 weeks （W） （ n = 5） groups. The OVX rats were subcutaneously injected with D - galaetose （15mg/100g） solution, once daily for 45 days （D）. Chronic pain model was established by ligating the left sciatic nerve with pieces of surgical suture. EA（2/ 15Hz 1- 2mA ） was applied to bilateral ＂Zusanli＂ （ ST 36） - ＂Yanglingquan＂ （ GB 34） for 30 min, once daily for 2D and 2 W, respectively. Hypothalamic Pre - proopiomelanocortin （ POMC ） mRNA expression, and hypothalamic and hippocampal prepronociceptin （PNOC） mRNA expression were detected by reverse transcription polymerase chain reaction （ RT - PCR）. Results：In comparison with the normal control group, the hypothalamic POMC mRNA expression was upregulated significantly （ P 〈 0. 05 ） and hypothalamus and hippocampal PPNOC mRNA expression downregulated considerably in CCI group （ P 〈 0. 05 ）. Compared with the CCI group, the expression of hypothalamic POMC mRNA was increased markedly in CCI ＋ EA2W group （P 〈 0. 05 ）, while the expression of hippocampal PNOC mRNA decreased distinctly in both CCI ＋ EA2D and CCI ＋ EA2W groups （P 〈 0. 05）. In OVX ＋ CCI rats, compared with the OVX ＋ CCI group, hypothalamic POMC mRNA expression was upregulated significantly in OVX ＋ CCI ＋ EA2W group （ P 〈 0. 05 ）, whereas hippocampal PNOC mRNA expression was downregulated remarkably （ P 〈 0.05）. No significant differences were found between the CCI and CCI ＋ EA2D groups, and between the OVX ＋ CCI and OVX ＋ CCI ＋ EA2D groups in the expression of hypothalamic POMC mRNA;between the CCI ＋ EA2D and CCI ＋ EA2W groups,between the OVX ＋ CCI and OVX ＋ CCI ＋ EA2D groups, and between the OVX ＋ CCI and OVX ＋ CCI ＋ EA2W groups in hypothalamic PPONC,and between OVX ＋ CCI ＋ EA2D and OVX ＋ CCI ＋ EA2W groups in hippocampal PNOC mRNA expression （P 〉 0.05 ）. Conclusion ：The cumulative analgesic effect of repeated EA of ST36 - GB34 is closely related to the upregulation of hypo-thalamic POMC expression and the downregulation of beth hippocampal and hypothhlamic PNOC mRNA expression.
出处
《辽宁中医杂志》
CAS
北大核心
2011年第3期445-449,共5页
Liaoning Journal of Traditional Chinese Medicine
基金
国家自然科学基金资助项目(30472241
30973796
90709031)
国家重点基础研究发展计划(973计划)资助项目(2007CB512505
2006CB504506
2005CB523306)
