摘要
【目的】探讨啤酒花品种青岛大花脱毒苗植株再生技术,以期获得青岛大花脱毒苗快速繁殖的便捷途径。【方法】以青岛大花脱毒苗的单芽茎段为外植体,分别以不同水质(自来水、凉开水、蒸馏水、当地井水)、不同前茬和简易营养液处理(带芽根茬、带芽根茬+简易营养液、新鲜培养基)及不同激素组合进行芽诱导和植株再生。【结果】在芽诱导及植株再生培养过程中,以当地井水进行外植体培养的效果较好,虽然其诱导芽数、新增芽数、增殖倍数稍低于对照蒸馏水,但生根茎段数、生根率、平均根长、茎粗和株高均最高,其次为凉开水处理;自来水处理的生根茎段数、诱导芽数、新增芽数、增殖倍数、株高等均明显低于蒸馏水对照。在前茬培养基上添加10mL简易培养液对外植体培养20d,其诱导芽数、新增芽数、增殖倍数、株高均明显高于新鲜固体培养基处理,且叶色均浓绿。在不同激素组合中,随着6-BA、IAA浓度的增加,单芽茎段的诱导芽数、生根率、增殖倍数呈先降低后增加的趋势,其中以原继代培养的激素组合6-BA0.1mg/L+IAA0.2mg/L的诱导芽数、生根率、增殖倍数最高,其次为激素组合6-BA0.01mg/L+IAA0.02mg/L。【结论】在外植体诱导和植株再生培养中,可用当地井水、凉开水作为培养基介质取代蒸馏水;继续利用前代培养基并留茬、再适量添加简易培养液对外植体进行培养,可有效提高脱毒苗的增殖倍数,且再生苗质量不受影响;可使用含低浓度激素组合(6-BA0.01mg/L+IAA0.02mg/L)进行脱毒试管苗芽诱导和植株再生培养,从而节省生产成本。
[Objective]The present experiment was conducted to study the regeneration technologies for virus-free plantlet of Humulus lupulus L. variety Qingdaodahua in order to develop a convenient method for its rapid propagation. [Method]The single budded stem of Humulus lupulus L. variety Qingdaodahua was used as explants to investigate the effects of cuhure medium containing water from different sources （tap water, cool boiling water, distilled water and local well water）, residual root stubble and simple nutrient solution （root stubble with single bud, root stubble ＋simple solution as MS medium without hormone, new culture medium）, and different hormone combinations （0.01-0.1 mg/L 6-BA and 0.02-0.2 mg/L IAA） on bud induction and shoot regeneration. [Result]The results showed that the effects of local well water on explants culture was better than the other water types, the higher rooting stem number, rooting rate, average root length, shoot diameter and height were observed in the medium containing water from the local well, however, the induction and prolifera- tion of buds and proliferation time were found to be lesser. This was followed by the medium containing cool boiling water. The rooting stem number, induction buds, proliferation buds and times, and shoot height of explants in tap water medium were lower than that of the control of distilled water. After culturing the explants for 20 days in residual root stubble medium＋20 mL simple solution, the induction and proliferation of buds, proliferation time and shoot height were found higher than those in new culture medium, and the leaves appeared on the shoots were in deep green. With the increase of 6-BA and IAA concentration in medium, the induction of buds, rooting rate and time of single bud proliferation decreased initially, and then registered an incease, and these were highest in the medium containing combination 6-BA （0.1 mg/L） and IAA （0.2 mg/L）. [Conclution]For the induction of buds in explant and regeneration of shoot, local well water or cool boiling water may be used as a substitute of distilled water in culture medium. The subculture medium and residual stubble can be used continuously to culture explants by adding simple solution （MS without hormone）. Lower concentrations of hormone combination 6-BA （0.01 mg/L）＋IAA（0.02mg/L） can be used to induce the buds and regeneration of shoot derived from virus-free plantlet of Humulus lupulus L. variety Qingdaodahua.
出处
《南方农业学报》
CAS
CSCD
2011年第2期128-130,共3页
Journal of Southern Agriculture
基金
甘肃省科技重大专项项目(092NKDH005)
特色农作物生物技术育种创新团队项目(098TTCH002)
关键词
啤酒花
青岛大花
脱毒苗
快繁
单芽茎段
Humulus lupulus L.
Qingdaodahua
virus-free plantlet
rapid propagation
single bud
