摘要
目的观察淫羊藿苷在体外试验中对α-突触核蛋白(α-synuclein,α-Syn)及相关蛋白表达的影响及其作用机制。方法 不同浓度的淫羊藿苷与转染α-Syn基因PC12细胞共孵育24h,应用RT-PCR法检测各组细胞α-Syn mRNA含量,Western免疫印迹法检测细胞中α-Syn、泛素化蛋白羧基端水解酶(Ubiquitin C-terminal hydrolase protein,UCH-L1)和Parkin蛋白的表达,观察淫羊藿苷对它们的影响。结果 淫羊藿苷浓度40～80μmol/L与转染α-Syn基因细胞孵育24h,能够明显减少模型细胞中α-SynmRNA含量,抑制Syn蛋白表达和聚集,增强UCH-L1和Parkin蛋白表达。结论 淫羊藿苷在体外对基因转染细胞的α-Syn过表达和聚集有明显抑制作用,其作用机制与抑制α-Syn合成和增强α-Syn降解有关。
Objective：To explore the effects of icariin,an effective component of Epimedium,on the over-expression and aggregation of α-synuclein（α-Syn） and other protein in vitro and mechanisms of the effects.Methods：Different concentrations of icariin were incubated with α-Syn gene-transfected PC12 cells for 24 h.The change in α-Syn mRNA content in the cells was determined by RT-PCR.The protein expression of α-Syn,UCH-L1 and Parkin was measured by Western blotting.Results：Incubation of icariin（40~80 μmol/L） with α-Syn gene-transfected PC12 cells for 24 h significantly decreased α-Syn mRNA content,inhibited α-Syn protein expression and aggregation,and enhanced the protein expression of UCH-L1 and Parkin in the model cells.Conclusion：Icariin inhibited the over-expression and aggregation of α-Syn in gene-tranfected PC12 cells in vitro,and the mechanisms may be related to the inhibition of synthesis and the enhancement of degradation of α-synuclein.
出处
《首都医科大学学报》
CAS
北大核心
2011年第1期79-83,共5页
Journal of Capital Medical University
基金
国家自然科学基金项目(30973513
30701092)
北京市自然科学基金项目(7072033)
北京市新世纪百千万人才工程项目(08-018)
北京市高层次卫生技术人才计划资助项目(2009-3-66)~~
