摘要
目的探讨哺乳期母鼠接触氯氰菊酯对雄性仔鼠断乳期及成年性激素合成的影响。方法将140只刚出生ICR小鼠随机分为氯氰菊酯染毒组和溶剂对照组,从分娩后第一天,染毒组每天给予母鼠25mg/kg氯氰菊酯灌胃染毒,直至分娩后第21天仔鼠断奶,对照组给予等体积玉米油灌胃。分别于出生后21天及70天时,每组取15只雄性仔鼠,放免法检测血清和睾丸组织睾酮(T)和雌二醇(E2)水平,用RT-PCR方法检测睾丸中StAR和睾酮合成酶mRNA表达水平,用Western blot检测睾丸中StAR和睾酮合成酶的蛋白表达水平。结果染毒组断乳期雄性仔鼠血清睾酮显著降低(P<0.01)及睾丸睾酮水平降低(P<0.05),对雌激素水平没有影响(P>0.05),且哺乳期氯氰菊酯暴露组雄性仔鼠睾丸中P450scc的mRNA水平与蛋白表达水平较对照组均显著降低(P<0.01),StAR、17β-HSD和P450 17α的mRNA表达水平较对照组有一定降低(P<0.05),但其蛋白表达水平几乎未受影响。哺乳期母鼠氯氰菊酯暴露对成年雄性仔鼠的血清睾酮、睾丸睾酮水平及睾丸中StAR和睾酮合成酶的mRNA与蛋白表达水平几乎没有影响(P>0.05)。结论哺乳期氯氰菊酯暴露可能主要通过下调睾丸中P450scc的mRNA与蛋白表达而影响21天雄性仔鼠睾丸中睾酮的合成。
Objective To discuss the influence on the testicular steroidogenesis synthesation during baby mice cut-milking period and adult period of male mice by the cypermethrin exposure during lactation of the female mice.Methods Fourteen dams of new-born mice were randomly divided into two groups,cypermethrin poisoning group and solution control group.Since the first day after baby delivery,the aternal mice in the cypermethrin poisoning group were given stomach cypermethrin poisoning,using the corn oil as the solution and the poison dose is 25mg/kg,until the 21st day after the delivery when the baby mice has cut-milking.While the control group is given the gavagy of the corn oil of the same volume.In each group,15 male baby mice were killed at 21st day and 70th day after delivery,respectively.Taking blood from the eye-balls,and separate the testes.Use radioimmunoassay（RIA） method to measure serum testosterone（T） and estrogen（E2） levels.Use RT-PCR method to measure StAR in the testes and the mRNA expression level of testosterone synthetic enzymes.Use Western blot to meausre StAR in the testes and the protein expression level of testosterone synthetic enzymes.Results Cypermethrin exposure during lactation of the female mice significantly leads to the decrease of serum testosterone of the male baby mice（P 0.01） and the decrease of the testosterone in the testes（P 0.05）,having no influence of the famale hormones（P 0.05）.And the cypermethrin exposure during lactation makes the significant decrease of the expression levels of the mRNA and the protein,of P450scc in the male baby mice testes,compared with the control group.And the mRNA expression levels of StAR,17β-HSD,and P450 17α decrease somewhat（P 0.05）,compared with the control group,but the protein expression levels of the above almost suffer no influence.Cypermethrin exposure during lactation of the female mice has almost no influence on the serum testosterone level,testosterone level in the testes,the StAR in the testes,and the expression levles of mRNA and protein of the testosterone synthetic enzymes（P 0.05）.Conclusion Cypermethrin exposure during lactation may influence testosterone biosynthesis in the testes of the 21 days male baby mice,mainly by down-modulating mRNA and protein expression levels of testicular P450scc.
出处
《卫生研究》
CAS
CSCD
北大核心
2011年第2期167-171,共5页
Journal of Hygiene Research
基金
安徽省高等学校省级自然科学研究项目(No.KJ2008B304)
教育部科学技术研究重点项目(No.208060)
关键词
氯氰菊酯
哺乳期
雄性仔鼠
睾酮
cypermethrin
lactation
male offspring
testosterone
biosynthesis
