摘要
目的:研究药用植物蛇足石杉内生真菌的分布及其多样性,并筛选具有乙酰胆碱酯酶抑制活性的内生真菌。方法:采用平板分离法分离蛇足石杉内生真菌,结合形态学和ITS-rDNA序列分析鉴定内生真菌;并采用DTNB显色法,对分离获得的内生真菌进行乙酰胆碱酯酶抑制活性的筛选。结果:共分离到127株内生真菌,分属于Penicillium,Aspergillus,Podospora等19个属;根、茎、叶中内生真菌的分布存在一定的组织差异性,茎中内生真菌的分离率和定殖率最高,叶中次之,根中最少。共有来自15个属的39株内生真菌显示出乙酰胆碱酯酶抑制活性,且11株有显著的抑制活性,其中7株来源于叶中。结论:药用植物蛇足石杉内生真菌多样性丰富,不同组织部位内生真菌的数量、种类及分布存在一定差异;同时蛇足石杉蕴含着大量抑制乙酰胆碱酯酶活性的内生真菌,为寻找和开发具有乙酰胆碱酯酶抑制活性的药用资源开辟了一条新的途径。
A total of 127 strains of endophytic fungi were isolated from roots,branches and leaves of Huperzia serrata.These strains were identified into 19 genera based on morphological characters and ribosomal DNA（rDNA） sequence analysis,thereinto Penicillium,Aspergillus and Podospora were dominant populations in H.serrata.From analysis results we found some endophytic fungi showed a certain degree of tissue preference.The isolation rate and colonization rate of stems were both larger than those of leaf and roots.After testing the acetylcholinesterase（AChE） inhibitory activity of these endophytic fungi,a total of 39 endophytic fungi belonging to 15 genera showed AChE inhibition.Eleven endophytic fungi showed potent AChE inhibition,7 of which were isolated from leaf.The research not only provided theoretical basis for developing and utilizing the resources of endophytic fungi in H.serrata but also showed a new path for searching medicines resource which has AChE inhibitory activity.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2011年第6期734-740,共7页
China Journal of Chinese Materia Medica
基金
国家自然科学基金项目(21066014)
江西省教育厅项目(GJJ11602,GJJ11393)
江西省科技厅重大项目(2010BSA19200)
江西师范大学研究生创新基金项目(YJS2010068)
关键词
蛇足石杉
内生真菌
分离
乙酰胆碱酯酶
抑制活性
Huperzia serrata
endophytic fungi
isolation
acetylcholinesterase
inhibitory activity
