摘要
采用细胞悬浮的方法，从4个烟草品种（系）中筛选出辅Q10含量较高的黄花大金元．以其悬浮培养细胞为材料，比较了几种植物生长调节剂对细胞生长和辅酶Q10含量的影响．结果表明，0．1～0．2mg／L的BA和KT对烟草细胞有显著的促进作用，但使辅酶Q10含量略有降低；0．5～8．0mg／L的2，4-D可显著提高辅酶Q10含量，但当其浓度高时对细胞生长不利；IAA和NAA对烟草细胞生长和辅酶Q10含量均无明显作用．此外，烟草悬浮培养细胞经丙酮抽提，石油醚萃取，通过硅胶柱层析获得辅酶Q10结晶，回收率达59.2％；用HPLC检测其纯度达97．7％．
A tobacco cell stain of HHDJY was selected by suspension cultare from 4 tobacco strains cultivated in South China on the level of CoQ10. By using this tobacco cell strain, the effectS of several plant growth regulation on the cell growth and content of CoQ10 were studied. The growth of the cells was greatly enhanced by 0.1 ~ 0. 2 mg/L BA and KT. 2,4-D could increase the content of CoQ10 but becampe unfavorable to cell growth when the concentration was high. IAA and NAA had no effect on cell growth and content of CoQ10. Morcover, CoQ10 crystal was obtained from suspension cells with acetone abstraction,petrolembenzine extraction and silica chromatography. The recovery of CoQ10 was 59. 2%, and the purity reached 97. 7 %.
出处
《华南农业大学学报》
CAS
CSCD
北大核心
1999年第4期59-64,共6页
Journal of South China Agricultural University
基金
广东省自然科学基金!960452
