摘要
目的 克隆人甲状腺特异性转录因子 - 1基因片段 ,为进一步从事分子甲状腺学研究打下基础。 方法 采用 RT- PCR法从人甲状腺组织 RNA中扩增出甲状腺特异性转录因子 - 1基因片段( 44 0～ 80 3bp) ;应用 TA克隆技术对 PCR产物进行分子克隆 ;Eco R I双酶解法鉴定重组体。 结果 获得 TTF- 1c DNA的阳性重组体 ( p CRTM / TTF- 1) ,经 Eco RI双酶解表明克隆成功。 结论 TTF- 1基因的分子克隆可进一步研究 TTF- 1基因对甲状腺特异蛋白质基因表达调控作用。
To obtain clones of thyroid specific transcription factor 1(TTF 1) gene \ Methods\ TTF 1 gene(440～803 bp) was amplified from human thyroid tissues RNA by RT PCR and cloning into PCR\+\{TM\} Ⅱ vector by TA cloning technique \ The resulting clones were digested by EcoR I \ Results\ Through the analysis of restrictive endonuclease,recombinant PCR\+\{TM\} Ⅱ/TTF 1 actually contained the cDNA sequence encoding TTF 1 \ Conclusion\ We successfully obtained recombinant PCR Ⅱ/TTF 1 \ This work provides an approach for further researches on mechanisms of regulation of TTF 1 on pathogenesis of thyroid diseases \;
出处
《福建医科大学学报》
1999年第3期248-250,共3页
Journal of Fujian Medical University
