摘要
以"红皮红肉"火龙果带腋芽茎段为外植体为试材,进行组织培养试验。结果表明:火龙果的最佳启动培养基为MS+5.0 mg/L 6-BA+0.05 mg/L NAA,诱导率为70%;增殖培养基为MS+6.0 mg/L 6-BA+0.09 mg/L NAA,增殖系数为3.5;生根培养基1/2MS+0.30 mg/LIBA+0.20 mg/L NAA,生根率达95%以上,平均生根条数为7.2条/株,根长长达8.2 cm,苗势较为旺盛。
Taking stem sections of axillary bud of Hylocereus undatus pitaya with stem as explant,using method of tissue culture were studied.The results showed that the best medium for start was MS＋5.0 mg/L 6-BA＋0.05 mg/L NAA,the induce rate was 70%;best medium for proliferation was MS＋6.0 mg/L 6-BA ＋ 0.09 mg/L NAA,proliferation coefficient was 3.5;best medium for rooting was 1/2MS＋ 0.30 mg/L IBA＋ 0.20 mg/L NAA,rooting rate more than was 95%,the average number of rooting was 7.2 bar/plant,the length of root was 8.2 cm,the seedling grew more exuberant.
出处
《北方园艺》
CAS
北大核心
2011年第5期167-169,共3页
Northern Horticulture
关键词
火龙果
组织培养
快速繁殖
Hylocereus undatus
tissue culture
rapid propagation
