摘要
背景:有研究表明在人类肝细胞癌中存在Hedgehog信号通路异常激活,但在动物肝细胞癌中Hedgehog信号通路是否激活尚不清楚。苦参可抑制肝癌细胞增殖周期,但其与Hedgehog信号通路的关系少见报道。目的:观察Hedgehog信号通路在人工诱导SD大鼠肝细胞癌中的作用,以及复方苦参药物对Hedgehog信号通路影响。方法:二乙基亚硝胺诱导SD大鼠建立肝细胞癌大鼠模型后,随机分为模型组、低剂量苦参组和高剂量苦参组,并设立空白对照组。建模后15周,分别在低、高剂量苦参组腹腔注射30,90mg/(kg·d)复方苦参治疗3周后,应用免疫组织化学染色和RT-PCR法检测这所有大鼠组织中在Hedgehog信号通路中靶基因转录因子Gli2和跨膜蛋白受体复合物Ptch的表达情况。结果与结论:相比对照组,模型组、低剂量和高剂量苦参组肝癌组织中Gli2和Ptch均呈高表达(P<0.01)。结果提示,在人工诱导的SD大鼠肝癌模型中,Hedgehog信号通路处于激活状态。在给予高低剂量复方苦参干预下,Hedgehog信号通路靶基因转录因子Gli2和跨膜蛋白受体复合物Ptch呈高表达。
BACKGROUND：Studies have demonstrated that Hedgehog signalling pathway is activated in human hepatocarcinoma,whether it is also activated in animal hepatocarcinoma remains unclear.Sophora flavescens Ait can inhibit proliferation cycle of hepatoma carcinoma cells.The correlation between Sophora flavescens Ait and Hedgehog signalling pathway remain poorly understood.OBJECTIVE：To study the role of hedgehog signaling pathway activation in artificially induced-SD rat hepatocarcinoma and to investigate the effect of compound radix sophorae flavescentis injection （CRSFI） on hedgehog signaling pathway.METHODS：SD rats were established diethylnitrosamine-induced hepatocarcinoma models,and then randomly divided into model group,low-dose CRSFI group and high-dose CRSFI group,at the same time,the control group was established.Rats in the low-and high-dose CRSFI groups were given the treatment of CRSFI concentration for 30 and 90mg/kg/d,for 3 weeks by intraperitoneal injection at 15 weeks after modeling.The expression of target gene transcription factors Gli2 and transmembrane protein receptor complex Ptch in the hedgehog signaling pathway was detected by immunohistochemical staining and RT-PCR.RESULTS AND CONCLUSION：The expression of Gli2 and Ptch was high expressed in the model,low-and high-dose CRSFI groups compared with the control group （P0.01）.The results suggest that the hedgehog signaling is abnormally activated in artificially induced-SD rat hepatocarcinoma.The expression of Gli2 and Ptch in Hedgehog signalling pathway is highly positive after treated by different dose CRSFI.
出处
《中国组织工程研究与临床康复》
CAS
CSCD
北大核心
2011年第2期257-260,共4页
Journal of Clinical Rehabilitative Tissue Engineering Research
基金
重庆市卫生局2008年度医学科研项目计划(渝08-02-40)
项目名称:肝癌Hedgehog信号通路的失调与复方苦参靶点干预~~
