摘要
目的探讨建立犬体外腹主动脉末端阻断动物模型的可行性,以供骨盆及以下部位创伤所致的动脉性大出血急救时临时控制出血进行研究。方法取杂种犬5只,麻醉后气管插管。应用自制体外腹主动脉阻断装置,自体外将腹主动脉远段向脊椎椎体挤压至血流完全阻断。以股动脉及肢体小动脉搏动消失为标准观察体外腹主动脉阻断压。观测犬心率(HR)、呼吸频率(RR)、中心静脉压(CVP)、前肢动脉收缩压(SBP)、舒张压(DBP)、平均动脉压(MAP)等指标变化,以及动物存活、麻醉清醒后肢体活动情况。结果单次阻断时间30分钟,体外腹主动脉末端阻断前后HR、RR、SBP、DBP、MAP、CVP总体基本平稳,阻断后上述指标均有轻度升高;动脉开放后SBP、DBP、MAP、HR则逐渐降至阻断前水平并趋于平稳,CVP先略有降低,30分钟后恢复至阻断前水平并趋于平稳;动脉开放30分钟后RR仍略高于阻断前水平。全部实验犬无意外死亡,实验后观察1周后无异常表现。结论犬体外腹主动脉末端阻断动物模型可成功建立,为探讨体外腹主动脉末端临时阻断控制动脉性大出血的止血效果和病理生理变化提供了手段。
Objective The study was designed to investigate the feasibility of establishing the dog model by blocking the end of abdominal aorta with extracorporeal approach so as to provide a research approach for temporally controlling the arterial hemorrhoea caused by fractures on the pelvis and the sites below the pelvis in emergency situation.Methods Tracheal intubation was performed after anesthetization and fore limb was cannulated for physical multifunction monitoring in five hybrid dogs.The extracorporeal appliance made by ourselves was used to press the distal of aorta abdominalis to the centrum until completely blocking the arterial blood flow,which was judged by whether the pulse of arteria cruralis and arteriola of lower limbs was disappeared.The pressure of complete blocking of the flow of aorta abdominalis was recorded.The physical signs changes of the dogs with HR,RR,CVP,SBP,DBP,MAP were observed.Also,the dogs＇ condition of survival and posterior limbs＇ movement after palinesthesia from the anesthesia was recorded.Results Pressure of blocking the end of abdominal aorta with extracorporeal approach was（429.90±47.32）mmHg.Time for single blockage was 30 minutes.The physical signs of HR,RR,SBP,DBP,MAP,CVP kept stable before and after blood flow blockage.All of these physical signs revealed slight elevation after blood flow blockage.However,when restoring the blood flow,physical signs of SBP,DBP,MAP,HR gradually returned to the level of pre-blockage and remained stable.CVP showed initially slight decrease.And then,30 minutes after stopping blocking blood flow,CVP returned to the level of pre-blockage and maintained stable.But the RR level was still higher than that of the pre-blockage.There was no death in the experimental dogs.Meanwhile,no abnormal manifestation was found 1 week after experiment.Conclusion The dog model of blocking the end of abdominal aorta by extracorporeal approach can be successfully established,providing a mean to investigate the effectiveness of hemostasis and pathophysiologic variation in temporally blocking the end of abdominal aorta by extracorporeal approach.
出处
《创伤外科杂志》
2011年第2期159-162,共4页
Journal of Traumatic Surgery
基金
南方医科大学南方医院院长基金(No:20060026)
南方医科大学新业务新技术课题(No:2007018)
关键词
骨盆骨折
止血
腹主动脉
模型
pelvic fracture
hemostasis
abdominal aorta
model
