摘要
目的:探讨草苁蓉苯丙素苷(PGBR)对肺癌细胞增殖的抑制作用及其可能机制。方法:采用MTT法观察PGBR对A549肺癌细胞增殖的影响,采用流式细胞术检测对A549细胞周期分布和细胞凋亡的影响,采用TUNEL染色法检测对A549细胞凋亡的影响,免疫细胞化学法检测凋亡相关蛋白p53,Fas,Bax,Bc1-2蛋白的表达。结果:PGBR可时间和浓度依赖性地抑制肺癌细胞增殖,200 mg.L-1 PGBR作用于A549细胞12,24,48 h时,抑制率分别为(20.4±2.1)%,(27.1±2.0)%,(30.7±2.2)%。PGBR改变肺癌细胞周期分布,使G0/G1期细胞由(45.3±4.1)%增至(57.4±4.0)%,表现出G0/G1期阻滞;同时诱导肺癌细胞凋亡,凋亡率由(2.4±0.90)%增至(7.8±1.2)%。PGBR明显增加p53和Fas表达,增加Bax表达和降低Bc1-2表达。结论:PGBR可通过诱导细胞周期阻滞和细胞凋亡而发挥抗肺癌作用。
Objective：To study the inhibitory effect and mechanism of phenylpropanoid glycosides from Boschniakia rossica（PGBR） on proliferation of lung carcinoma cells.Method： MTT assay was used to examine the proliferation of A549 cells,flow cytometry was used for the determination of cell cycle distribution and cell apoptosis,TUNEL method was used for the determination of cell apoptosis,and immunohistochemical method was used for the determination of p53,Fas,Bax and Bcl-2 protein expression.Result： PGBR inhibited the proliferation of lung carcinoma cells in a time-and concentration-dependent manner,the inhibitory ratios of 200 mg.L-1 PGBR at 12,24 and 48 h were（20.4 ± 2.1）%,（27.1 ± 2.0）% and（30.7 ± 2.2）%,respectively.PGBR increased the percentage of G0 /G1 phase cells from（45.3 ± 4.1）% to（57.4 ± 4.0）%,and increased the apoptotic ratio from（2.4 ± 0.90）% to（7.8 ± 1.2）% to（57.4 ± 4.0）%,and increased the apoptotic ratio from（2.4 ± 0.90）% to（7.8 ± 1.2）%.Furthermore,PGBR increased the expression of p53,Fas and Bax proteins and decreased the expression of Bcl-2.Conclusion：PGBR could exert an anti-lung cancer effect via induction of cell cycle arrest and cell apoptosis.
出处
《中国实验方剂学杂志》
CAS
北大核心
2011年第7期213-216,共4页
Chinese Journal of Experimental Traditional Medical Formulae
基金
吉林省科技发展计划项目(200705428)
吉林省中医药管理局科技项目(08SYS-105)
关键词
草苁蓉
苯丙素苷
肺癌
增殖
凋亡
细胞周期
Boschniakia rossica
phenylpropanoids
lung cancer
proliferation
apoptosis
cell cycle
