摘要
芦丁和槲皮素在紫外区的最大吸收波长接近,吸收光谱严重重叠,普通光度法分析难以实现两者的直接测定。该文通过测定以1∶4甲醇-水为溶剂的芦丁、槲皮素标准混合溶液在200～500 nm波长范围的吸收光谱,采用偏最小二乘法(PLS法)建立校正模型,对样品中的芦丁和槲皮素的含量进行预测,建立了偏最小二乘-分光光度法同时测定芦丁和槲皮素含量的新方法。方法应用于槐米样品中芦丁、槲皮素含量的同时测定,其回收率分别为95.6%～99.8%、99.8%～99.9%;应用于模拟样中芦丁、槲皮素含量的同时测定,两者回收率分别为97.6%～102.1%、100.2%～102.2%。方法显示了较高的准确度和较好的精密度,且能够降低噪声对校正结果的影响。
The maximum absorption wavelengths of rutin and quercetin are very close and their absorption spectra are seriously overlapping, so it＇s very difficult to determine individually rutin or quercetin by speetrophotometry without prior separation. In this paper, a partial least squares - speetro- photometry was developed for the simultaneous determination of rutin and quereetin. The absorption spectra of rutin and quercetin mixed standard were studied in the range of 200 - 500 nm and the absorbanee values were recorded every 2 nm. A partial least-squares（PLS） model was built for the simuhaneous determination of the contents of rutin and quereetin in the flos sophorae and synthetic samples. The results indicated that the recoveries of rutin and quercetin in the flos sophorae were in the range of 95.6% - 99. 8% and 99.8% - 99.9% , respectively, and the recoveries of rutin and quercetin in the synthetic samples were in the range of 97.6% - 102. 1% and 100. 2% - 102.2% , respectively. The method showed high accuracy and satisfied precision, and could reduce the effect of noise effectively.
出处
《分析测试学报》
CAS
CSCD
北大核心
2011年第4期457-460,共4页
Journal of Instrumental Analysis
基金
国家自然科学基金资助项目(20975081)
西北大学研究生交叉学科资助项目(07YJC09)
陕西省教育厅科研计划资助项目(2010JK899)
咸阳师范学院专项科研基金资助项目(10XSYK205)
教育部科学技术研究重点资助项目(204182)
关键词
偏最小二乘-分光光度法
芦丁
槲皮素
同时测定
partial least square spectrophotometry
rutin
quercetin
simultaneous determination
