摘要
[目的]研究一氧化氮(NO)促进汞胁迫下小麦种子的萌发及其对萌发过程中氧化胁迫的缓解效应。[方法]采用不同浓度的HgCl2模拟重金属汞胁迫梯度处理小麦种子,以小麦种子发芽率达到对照的50%时,为汞胁迫对小麦种子萌发的半抑制浓度;以硝普钠(SNP)作为NO的供体,预处理小麦种子12 h后,置于半抑制浓度的汞胁迫条件下,研究小麦种子的发芽状况,探查种子中淀粉酶和蛋白水解酶的活性变化,以及过氧化氢酶(CAT)活性、脯氨酸和丙二醛(MDA)含量3个抗氧化指标的变化。[结果]NO供体SNP能显著促进汞胁迫下小麦种子的萌发,经SNP预处理的小麦种子中淀粉酶和蛋白水解酶的活性明显高于对照;SNP预处理能明显促进汞胁迫下小麦种子中CAT活性的上升,上调脯氨酸的水平,降低脂质过氧化产物MDA的含量。[结论]NO供体SNP能促进小麦种子萌发与幼苗的生长,并提高小麦种子的抗氧化能力。
[Objective] The aim was to study the effects of Nitric oxide（NO） on wheat seeds germination and its roles in reducing the oxidative stress.[Method] Different concentrations of HgCl2 was used to simulate the Hg2＋ stress to treat wheat seeds,the hemi-inhibitory concentration of Hg2＋ stress was achieved when the germination rate of wheat seeds reached 50% of the control;under the SNP pretreatment for 12 h and hemi-inhibitory concentration of Hg2＋ stress,the germination status of wheat seeds,the change of its protease and amylase activities,as well as the change of its CAT activity,proline and MDA content were studied.[Result] SNP could obviously the germination of wheat seeds under Hg2＋ stress,and the amylase and prolease activities of the SNP pretreated seeds were significantly higher than that of the control;SNP pretreatment could obviously increase the CAT activity of wheat seeds under Hg2＋ stress,improve the proline level and reduce MDA content.[Conclusion] SNP could promote the germination of wheat seeds and growth of wheat seedlings as well as improve its antioxidant capacity.
出处
《安徽农业科学》
CAS
北大核心
2011年第9期5161-5163,共3页
Journal of Anhui Agricultural Sciences
基金
江苏省科技成果转化专项(BA2008032)
江苏省农业科技自主创新项目[CX(09)635]
关键词
一氧化氮
汞胁迫
小麦种子萌发
抗氧化
Nitric oxide
Hg2＋ stress
Wheat seed germination
Antioxidation
