摘要
目的:建立高效液相色谱法测定"网脱Ⅰ号"口服液中阿魏酸的含量。方法:采用Diamonsil C18柱(150 mm×4.6 mm,5μm),以乙腈-1%冰醋酸(17∶83)为流动相,流速为1.0 mL·min-1,检测波长为300 nm。结果:阿魏酸在10～50μg·mL-1范围内呈良好的线性关系(r=0.999 36);平均回收率为100.86%,RSD=1.92%。结论:所建方法简便快速、准确、重复性好,可用于"网脱Ⅰ号"口服液的质量控制。
Objective：To establish an HPLC method for determining the content of ferulic acid in ＂NoⅠDetachment of Retina＂ oral solution.Methods：A Diamonsil C18 column（150 mm×4.6 mm,5 μm） was used for separation.The mobile phase consisted of acetonitrile-1% glacial acetic acid（17：83） at the flow rate of 1 mL·min-1,and the detection wavelength was set at 300 nm.Results：The linear range of ferulic acid was 10-50 μg·mL-1（r=0.999 36）,with average recovery rate of 100.86%,and RSD of 1.92%.Conclusion：The method was simple,fast,accurate and reproducible,and it can be used for the quality control of ＂NoⅠDetachment of Retina＂ oral solution.
出处
《中国药物应用与监测》
CAS
2011年第2期86-88,共3页
Chinese Journal of Drug Application and Monitoring
基金
中国人民解放军兰州军区科研项目(LXH–2008018)
