摘要
目的探讨甘露醇对骨髓间充质干细胞（BMSCs）移植治疗血管性痴呆（VD）大鼠行为学及海马胆碱能系统活性的影响。方法体外全骨髓培养结合细胞贴壁法培养大鼠BMSCs。采用双侧颈总动脉结扎法制备VD模型。将大鼠随机分为4组：（1）甘露醇预处理BMSCs组：造模4w后,先进行尾静脉注射20%甘露醇,10~30m in后,再尾静脉注射BMSCs;（2）BMSCs组：造模4w后,经尾静脉注射等量BMSCs;（3）培养基组：造模4w后,经尾静脉注射等量BMSCs基础培养基;（4）假手术组：不进行任何干预。BMSCs移植4w后进行Morris水迷宫试验和大鼠海马内胆碱乙酰转移酶（ChAT）和乙酰胆碱酯酶（AChE）活性检测。结果甘露醇预处理BMSCs组的ChAT和AChE活性均比BMSCs组、培养基组有明显提高（P〈0.05）,同时其行为学亦较BMSCs组、培养基组有明显改善（P〈0.05）。结论甘露醇预处理后进行静脉注射移植BMSCs,使VD大鼠模型海马胆碱能系统的活性明显增强,并进一步改善VD大鼠的学习与记忆能力。
Objective To explore the change of the ethology and cholinergic system of the hippocampus formation by intravenous administration of bone marrow mesenchymal stem cells（BMSCs）with mannitol among vascular dementia（VD） rats.Methods The BMSCs of rat were isolated and cultured by whole bone marrow adherence screening method.The models of VD rats were established through permanent ligation of bilateral common carotid arteries at an interval of 3 days for each artery.The healthy matured-male SD rats were divided randomly into four group.（1）Mannitol with BMSCs group： intravenous injection of mannitol at a dose of 1.5g/kg followed 10~30minutes later by intravenous injection of 1 million BMSCs in 1ml PBS at the fourth week after ligation.（2）BMSCs group：intravenous injection of 1 million BMSCs in 1ml PBS at the same time.（3）PBS control group：intravenous injection of only 1ml PBS.（4）Sham group：no ligation and no intravenous injection.the ethology and The activity of choline acetyltransferasecholine（ChAT） and acetycholinesterase（AChE） were tested at the fourth week after injection.Results The activity of ChAT and AchE significantly improved in the hippocampus of rats in Mannitol with BMSCs group compared with BMSCs group and PBS control group（P0.05）,so did the ethology.The same significant improve in BMSCs group compared with PBS control group（P0.05）.Conclusions BMSCs improve the ethology and cholinergic system activity of the hippocampus formation of VD rats.The beneficial effects of intravenous injection of BMSCs are amplified with intravenous injection of mannitol.
出处
《中风与神经疾病杂志》
CAS
CSCD
北大核心
2011年第4期317-320,共4页
Journal of Apoplexy and Nervous Diseases
基金
广西科学研究与技术开发计划课题(桂科攻0632007-1C)
