摘要
目的:研究十堰市霍乱弧菌分子生物学特征,为疫情分析,预警及制定防控策略提供科学依据。方法:对3株霍乱弧菌分离株进行MPCR毒力基因检测,12种常用药物的药敏试验,并送中国疾病预防控制中心传染病所进行PFGE同源性分析。结果:3株霍乱弧菌均为不产毒素的非流行株;PFGE同源性分析表明2008年从猪瘦肉和基围虾种分离到的两株O1群霍乱弧菌为同一来源,他们对阿莫西林/克拉维酸、链霉素中度敏感,对磺胺复合物耐药;2009年从污水中分离到的1株O139群霍乱弧菌对环丙沙星中度敏感,对复方新诺明耐药。结论:这3株霍乱弧菌均为不产毒素的非流行株。
Objective：To study the molecular biological characteristic of Vibrio cholerae isolated from Shiyan city,provide scientific basis for epidemic analysis,early warning and formulating prevention and control strategy.Methods： Three strains of V.cholerae isolates were detected for toxic genes by MPCR,durg-resistance assay for 12 antibiotics were carried out,and homology analysis by PFGE was finished in China Center for Disease Prevention and Control.Results： The three isolates of V.cholerae were all non-prevalent strains,without toxic genes.The result of homology analysis indicated that two stains of O1 V.cholerae isolated from meat and shrimp belonged to the same source,they were intermediate sensitive to amoxicillin/clavulanic acid and streptomycin,and resistant to sulfamethoxazole.One stain of V.cholerae O139 isolated from sewage was intermediate sensitive to ciprofloxacin,and resistant to sulfamethoxazole.Conclusion： These three isolates of V.cholerae were all the non-prevalent strains.
出处
《中国卫生检验杂志》
CAS
2011年第4期1008-1009,1011,共3页
Chinese Journal of Health Laboratory Technology
关键词
霍乱弧菌
同源性
药敏特征
Vibrio cholerae
Homology
Drug-resistant
