摘要
[目的]优化根癌农杆菌介导烟草K326遗传转化体系。[方法]以烟草无菌苗的叶片为外植体,通过农杆菌介导法对其遗传转化体系进行了优化研究。[结果]外植体在MS+2 mg/L6-BA+0.2 mg/L IAA培养基上进行2 d预培养后,用农杆菌GV3101(浸染浓度为OD600=0.6)浸染5 min,转化效率最高,达63%;经过PCR检测初步证明npt II基因已整合到烟草再生植株中。[结论]该研究建立了高效烟草K326叶片农杆菌介导的遗传转化体系。
[Objective] The aim was to optimize tobacco K326 genetic transformation system mediated by Agrobacterium.[Method] The leaf of tobacco aseptic seedling was taken as explants to study the optimization of K326 genetic transformation system via Agrobacterium.[Result] The highest transformation efficiency was obtained when the explants was pre-cultured in the medium of MS ＋ 2 mg/L 6-BA ＋ 0.2 mg/L IAA for 2 d,and then infected with Agrobacterium GV3101（OD600 = 0.6） for 5 min.The PCR detection proved that NPTII gene had been integrated into the regenerated tobacco plants.[Conclusion] A highly efficient genetic transformation system of tobacco leaf mediated by Agrobacterium was established.
出处
《安徽农业科学》
CAS
北大核心
2011年第8期4436-4437,4449,共3页
Journal of Anhui Agricultural Sciences
关键词
根癌农杆菌
烟草
遗传转化
Agrobacterium tumefaciens
Tobacco
Genetic transformation
