摘要
目的研究仙鹤草醋酸乙酯有效部位(总鞣质质量分数为19.91%)对人肝癌HepG2细胞增殖的抑制作用及其机制。方法以不同质量浓度的仙鹤草醋酸乙酯有效部位作用于体外培养的HepG2细胞,MTT法检测细胞生长抑制率;流式细胞仪检测细胞凋亡;Fluo-3/AM荧光探针观察肿瘤细胞内钙离子浓度的变化;流式细胞仪检测肿瘤细胞内活性氧(ROS)的变化。结果仙鹤草醋酸乙酯有效部位可显著抑制HepG2细胞的生长,诱导细胞凋亡,其IC50为127.85μg/mL。经该有效部位作用48h后,HepG2细胞数量明显减少;在Fluo-3/AM荧光探针的作用下有较强的绿色荧光;同时检测出细胞内ROS有较明显的增加。结论仙鹤草醋酸乙酯有效部位能抑制HepG2细胞的生长,诱导细胞发生凋亡。肿瘤细胞内钙离子的释放和细胞内ROS的增加可能是其作用机制。
Objective To study the antiproliferation effects of extract from Agrimonia pilosa （tannin 19.91%） on hepatocellular carcinoma HepG2 cells and their mechanisms. Methods HepG2 cells in culture medium were treated with different concentrations of extract ofA. pilosa. The inhibitory rate of the cells was measured by MTT assay. Cell apoptotic rate was detected by flow cytometry （FCM）. Fluo-3/AM fluorescent probe was used to observe the Ca2＋ variation in the tumour cells. The variation of intracellular ROS was detected by FCM. Results The extract ofA. pilosa could inhibit the growth of HepG2 cells and cause apoptosis significantly. The ICs0 was 127.85 μg/mL. The number of cells was decreased especially after the cells were treated 48 h by the extract ofA. pilosa, and the green fluorescence was seen clearly by Fluo-3/AM fluorescent probe. The intracellular ROS was increased obviously. Conclusion The extract ofA. pilosa could inhibit the growth of HepG2 cells and cause apoptosis. The releasing of the intracellular Ca2＋ variation and the increasing of the intracellular ROS may he its anti-hepatocellular carcinoma mechanisms.
出处
《现代药物与临床》
CAS
2011年第2期119-122,共4页
Drugs & Clinic
基金
黑龙江省留学回国人员重点科技项目(黑人发[2009]23号)
