摘要
采用FISH-AFLP技术,选取8对E+3和M+3引物组合,对云南省林木品种审定委员会审(认)定的17个核桃品种(无性系)进行了基因组DNA分子水平上的检测,实验结果共获得987条可统计的条带,其中942条呈多态性,多态性带百分率达95.44%;17份供试核桃样品的遗传相似系数范围为0.606 9 0.796 4,平均为0.734 9。本研究分析了云南省审(认)定核桃品种(无性系)的遗传多样性和亲缘关系,建立了它们的指纹图谱,为下一步的核桃新品种选育、构建核桃优良种质库提供了理论指导。
The genetic diversities of 17 walnut cultivars（Juglans sigillata Dode） authorized or approved by Yunnan Province Forest Tree Cultivar Registration Committee were investigated with FISH-AFLP technical system.Eight pairs of E＋3/M＋3 primers were used to amplify the genomic DNA.The results showed that 987 AFLP bands were obtained,942 of them were polymorphic markers and the average percent of polymorphic bands was 95.44%.The genetic similarity indexes among all the 17 cultivars are ranged from 0.606 9 to 0.796 4 with an average of 0.734 9.These investigations would be significant in utilizing walnut germplasm resources and cultivating new varieties.
出处
《林业科学研究》
CSCD
北大核心
2011年第2期189-193,共5页
Forest Research
基金
林业公益性行业科研专项经费项目:核桃优异种质资源保存与开发利用技术研究(200704046),核桃集约及可持续生产关键技术研究(201004048)
关键词
核桃
品种
遗传多样性
FISH-AFLP
聚类分析
walnut（Juglans sigillata Dode）
cultivar
genetic diversity
FISH-AFLP
cluster analysis
