摘要
背景:目前,纯钛及其合金因其较好的生物相容性及力学性能成为最常用的牙科植入体,但其本身并无抗菌性。目的:采用放电法处理钛板,观察其抗菌活性和生物相容性。方法:通过放电加工法制备钛板,以链球菌的变种培养,以菌落形成评估抗菌活性。通过造骨细胞和纤维母细胞在钛样本上的培养,观察细胞附着和繁殖情况并计算细胞数。并观察细胞结合蛋白吸收,评估钛板的组织相容性。进一步用电子显微镜的扫描图像观察样本表面结构,以薄层的X射线衍射来评估表面抗菌性能。结果与结论:经与未行表面处理的钛板对照比较,在氯化钠、氯化钾和氯化镁处理过的样本表现出抗菌活性,在1mol/L溶液处理后的样品细菌数明显减少,而在硫酸钠、乙酸钾和硫酸镁处理的样本则无抗菌活性。氯化钛显示更多的蛋白吸收。X射线衍射钛出现高峰,氧化钛表面稳定。检测显示三氯化钛变成了次氯酸,后者是有抗菌活性的。结果提示,用放电技术处理钛板表面方法。氯化钛样本即具有优良的细胞相容性,又有对口腔细菌的抗菌活性。
BACKGROUND：At present,pure titanium and its alloys have become the most commonly used dental implants because of its good biocompatibility and mechanical properties,but it is not anti-bacterial.OBJECTIVE：To examine the antibacterial activity and biocompatibility of arc-discharge processed titanium plates.METHODS：Titanium plates were prepared by electrical discharge.Antibacterial activity of the cultured streptococcus mutants was evaluated by colony formation.Osteoblasts and fibroblasts were cultured onto the titanium plates.Cell adhesion and proliferation were examined and cell numbers were counted.Absorption of cell binding protein was determined and tissue biocompatibility of the titanium plates was evaluated.Electron microscope scanning images were obtained to further examine sample surface microstructures.Lamellar X-ray diffraction was used for assessment of the surface anti-bacterial properties.RESULTS AND CONCLUSION：Samples treated in electrolyte solution containing sodium chloride,potassium chloride and magnesium chloride demonstrated antibacterial activity when compared to titanium plates without surface treatment.The number of bacterial colonies decreased significantly for samples treated in 1mol/L electrolyte solution,while samples treated in solution containing sodium sulfate,potassium acetate and magnesium sulfate showed no antibacterial activity.The assay result of cell binding protein absorption indicated more protein absorption for titanium chloride.The X-ray diffraction produced a titanium peak as parent metal,while the surface of titanium oxide was stable.Further analysis indicated that titanium trichloride was transformed into hypochoric acid with antibacterial activity.Titanium chloride samples demonstrated both excellent cellular biocompatibility and antibacterial activity against oral bacteria.
出处
《中国组织工程研究与临床康复》
CAS
CSCD
北大核心
2010年第51期9563-9566,共4页
Journal of Clinical Rehabilitative Tissue Engineering Research
基金
天津市高等学校科技发展计划项目(20060123)~~
