摘要
目的观察沙棘总黄酮对血管内皮细胞的保护作用。方法 采用人脐静脉内皮细胞系ECV304,以过氧化氢(H2O2)制备血管内皮细胞损伤模型;采用流式细胞仪测定内皮细胞凋亡率和平均荧光强度的变化;采用Westernblot法测定内皮细胞Caspase-3活性。结果 200μmol/L的H2O2可导致内皮细胞凋亡;不同浓度(400、200、100μg/mL)的沙棘总黄酮均显著降低H2O2所致内皮细胞凋亡率(P<0.01),并可明显抑制H2O2损伤内皮细胞Caspase-3活性(P<0.01)。结论 沙棘总黄酮对抗H2O2所致的内皮细胞凋亡;降低Caspase-3表达,是其内皮细胞保护作用机制之一。
Objective To observe the protection of total flavones of Hippophae Rhamnoides（TFH） on vascular endothelial cells（VECs）.Methods Human umbilical VECs（ECV304） were used.The vascular endothelial injured cell model was prepared using hydrogen dioxide（H2O2）.The cell apoptosis rate and changes of mean fluorescence intensity were detected using flow cytometry（FCM）.The Caspase-3 activity in VECs was detected by Western blot.Results VEC apoptosis was induced by 200 μmol/L H2O2.TFH in different concentrations（400,200,and 100 μg/mL） could significantly lower the cell apoptosis rate induced by H2O2 respectively（all P0.05）,and obviously inhibit Caspase-3 activities（all P0.01）.Conclusions TFH could fight against H2O2 injured VECs apoptosis.Lowering the Caspase-3 expression was one of its mechanisms in protecting VECs.
出处
《中国中西医结合杂志》
CAS
CSCD
北大核心
2011年第3期355-358,共4页
Chinese Journal of Integrated Traditional and Western Medicine
基金
辽宁省科学技术计划项目(No.2005408001)
关键词
沙棘总黄酮
血管内皮细胞
CASPASE-3
total flavones of Hippophae Rhamnoides
vascular endothelial cell
Caspase-3
