摘要
目的:研究C-反应蛋白(CRP)对培养的人外周血单核细胞抵抗素mRNA和蛋白表达的影响。方法:分离培养人外周血单核细胞,分为浓度效应组和时间效应组,即不同浓度的CRP(0,5,10,25,50μg/ml)刺激24h以及25μg/ml CRP刺激不同的时间(0,3,6,12,24h),分别用实时定量PCR和ELISA方法检测抵抗素的表达。结果:CRP可呈剂量依赖性和时间依赖性地诱导人单核细胞抵抗素mRNA和蛋白表达。结论:CRP可诱导人单核细胞抵抗素mRNA和蛋白表达。
Objective：To assess the possible effects of CRP on the expression of resistin by cultured human monocytes.Method：Human monocytes were incubated with varying concentrations of CRP（0,5,10,25 and 50 μg/ml,n=6 for each） for 24 h,and human monocytes were incubated with 25 μg/ml of CRP for different time（0,3,6,12,24,n=6 for each point respectively）.Then RT-PCR and ELISA were used to observe the resistin expression in human monocytes.Result：CRP induced the expression of resistin in cultured human monocytes in a dose and time-dependent manner.Conclusion： CRP could induce the expression of resistin in human monocytes.
出处
《临床心血管病杂志》
CAS
CSCD
北大核心
2011年第3期227-229,共3页
Journal of Clinical Cardiology
