摘要
目的:探讨海洛因戒断、脱毒及复吸期间大鼠直肠内5-羟色胺(5-HT)、P物质(SP)表达的改变.方法:正常♂SD大鼠,随机分为实验组、盐水对照组和正常对照组,实验组分别建立海洛因依赖戒断模型,美沙酮脱毒治疗模型和海洛因复吸模型.取各组大鼠直肠组织,采用免疫组织化学SABC法及图像分析方法进行研究.结果:与正常对照组及盐水对照组比较,实验组大鼠直肠内的5-HT、SP细胞免疫染色加深;图像分析5-HT、SP阳性细胞的细胞计数增多(5-HT:10d:5.09±3.39vs3.16±2.05,2.80±2.13;24d:5.05±3.04vs3.16±2.05,2.81±1.85;SP:10d:2.55±1.35vs1.29±0.86,1.37±0.93;24d:2.57±1.27vs1.29±0.86,1.39±0.79,均P<0.05),平均灰度值低于正常及盐水对照组(F=36.642,4.583,P<0.05).实验组与正常及盐水对照组比较,5-HT、SP阳性细胞免疫染色、细胞计数及平均灰度值变化差异均无统计学意义(均P>0.05).结论:5-HT、SP在大鼠直肠黏膜表达的改变,提示二者参与海洛因戒断、脱毒和复吸期间消化系功能恢复的调节过程.
AIM：To investigate the expression of 5-hydroxy-tryptamine（5-HT） and substance P（SP） in the rectum of rats during heroin abstinence,detoxication,and relapse.METHODS：Male Sprague-Dawley rats were divided into normal control group（NCG）,saline control group（SCG）,and experiment group（EG）.The EG group was further divided into heroin abstinence group（HAG）,methadone detoxication group（MDG）,and heroin relapse group（HRG）.Rectum tissue samples were taken from each group to perform immunohistochemistry to detect the expression of 5-HT and SP.RESULTS：Compared with the NCG and SCG groups,the immunostaining density of 5-HT-and SP-positive cells was greater,the numbers of 5-HT-and SP-positive cells increased（5-HT：10 d：5.09 ± 3.39 vs 3.16 ± 2.05,2.80 ± 2.13;24 d：5.05 ± 3.04 vs 3.16 ± 2.05,2.81 ± 1.85;SP：10 d：2.55 ± 1.35 vs 1.29 ± 0.86,1.37 ± 0.93;24 d：2.57 ± 1.27 vs 1.29 ± 0.86,1.39 ± 0.79,all P 0.05）,and the mean grey degree of 5-HT-and SP-positive cells decreased（F = 36.642,4.583,P 0.05） in the HAG and HRG groups.Compared with the NCG and SCG groups,there were no statistical significances in the immunostaining density,numbers,and mean grey degree of 5-HT-and SP-positive cells in the MDG group（all P 0.05）.CONCLUSION：Altered expression of 5-HT and SP in the tunica mucosa recti of rats during heroin abstinence or relapse suggests that 5-HT and SP can regulate the recovery of digestive function during heroin abstinence,detoxification,and relapse.
出处
《世界华人消化杂志》
CAS
北大核心
2011年第5期477-482,共6页
World Chinese Journal of Digestology
基金
贵州省社会发展科技攻关计划基金资助项目
No.黔科合S字(2007)1048~~
