摘要
采用乙酸乙酯漂浮方法和酸溶解法分别解决了再生水进水中有机杂质以及再生水中剩余絮凝剂干扰隐孢子虫和贾第鞭毛虫检测的关键问题,在此基础上,建立了成本远低于EPA 1623法的隐孢子虫和贾第鞭毛虫检测方法的密度梯度分离/免疫荧光技术。利用该方法对我国北方2个城市的污水再生处理系统进行了检测,再生水进出水的回收率分别为18%~31%卵囊和24%~95%包囊,能够满足检测要求。结果表明,在5个再生水系统的9个进水样品中,隐孢子虫的阳性率为89%,平均浓度为26个/10 L;贾第鞭毛虫的阳性率为100%,平均浓度为138个/10 L。再生水出水中也有两虫检出。
For the reclaimed influents and the reclaimed effluents with excess coagulant, the ethyl acetate floatation and acid dissociation were suggested to be added to enhance the purification and increase the recovery, respectively. Then an immunofluorescence assay and density gradient separation method for detection of Cryptosporidium oocysts and Giardia cysts, was developed in reclaimed wastewater systems. The Cryptosporidium oocysts and Giardia cysts of reclaimed wastewaters were detected in T and B cities in north China using immunofluorescence assay and density gradient separation method. The acceptable recoveries obtained from reclaimed influents and effluents were in the range of 18% -31% oocysts and 24% -95% cysts. The results showed that Cryptosporidium were detected in 89% samples with mean concentration of 26 ooeysts/10 L,while Giardia were detec- ted in 100% samples with mean concentration of 138 cysts/10 L in the influents of 5 reclaimed wastewater systerns. Besides, Cryptosporidium oocysts and Giardia cysts were also present in the effluents of some reclaimed wastewater systems.
出处
《环境工程学报》
CAS
CSCD
北大核心
2011年第5期982-986,共5页
Chinese Journal of Environmental Engineering
基金
国家"水体污染控制与治理"科技重大专项(2008ZX07314-003008)
国家自然科学基金资助项目(50778171)
关键词
再生水
隐孢子虫
贾第鞭毛虫
密度梯度分离
reclaimed water
Cryptosporidium
Giardia
density gradient separation
