摘要
目的:了解中山大学附属东华医院2008年临床常见病原菌对常用抗菌药物的耐药情况,为临床合理用药提供参考依据。方法:纸片扩散法(Kirby-Bauer)检测临床分离菌对各种抗菌药物的耐药性,参照2008版CLSI标准判定药敏结果,并用WHONET5.4软件进行统计分析。结果:全年共检出细菌3 594株,其中革兰阳性菌占39.87%,革兰阴性菌占49.67%。前5位的细菌依次为:大肠杆菌、肺炎克雷伯菌、铜绿假单胞菌、金黄色葡萄球菌和凝固酶阴性葡萄球菌。对抗生素耐药性检测结果显示,MRSA检出率为26%,MRCNS检出率为80%;未发现耐万古霉素的葡萄球菌;粪肠球菌和屎肠球菌对高浓度的庆大霉素的耐药率分别为46.2%和48.1%,1株粪肠球菌对万古霉素不敏感,各种肠道杆菌均对亚胺培南敏感;铜绿假单胞菌耐亚胺培南的菌株占15.4%。结论:我院住院患者分离的细菌耐药性比较严重,对多数常用抗菌药物耐药率呈上升趋势。
OBJECTIVE： To investigate the resistance of the common clinical pathogens to commonly used antibiotics in our hospital in 2008 for references of clinical rational drug use.METHODS： Antimicrobial susceptibility test was carried out by Kirby-Bauer method,and the results of the drug susceptibility test were assessed in accordance to CLSI（version 2008） criteria wiht WHONET 5.4 software used for statistical analysis.RESULTS： A total of 3 549 strains of pathogens were isolated from various clinical specimens in 2008,with gram positive cocci and gram negative bacilli accounting for 39.87% and 49.67%,respectively,leading the top 5 places were E.coli,K.pneumoniae,P.aeruginosa,S.aureus and coagulase negative staphylococcus.The antimicrobial resistance test showed a detection rate of 26.0% for MRSA（methicillin resistant staphylococcus aureus） and 80% for MRCNS（methicillin-resistant coagulase negative Staphylococcus）.No vancomycin-resistant staphylococcus strain was noted.The resistance rates of E.faecalis and E.faecium to high concentration of gentamicin（120 μg） were 46.2% and 48.1%,respectively.One E.faecalis strain was noted to be insensitive to vancomycin.Isolates of Enterobacteriaceae were all susceptible to imipenem.15.4% of P.aeruginosa strains were resistant to imipenem.CONCLUSION： The bacterial drug resistance in the clinical isolates from our hospital is serious with increasing resistance to most antimicrobial agents.
出处
《中国医院用药评价与分析》
2011年第4期350-352,共3页
Evaluation and Analysis of Drug-use in Hospitals of China
关键词
耐药性监测
抗菌药物
革兰阴性杆菌
革兰阳性球菌
Bacterial resistance surveillance
Antimicrobial agent
Gram negative bacilli
Gram positive cocci
