摘要
目的:探讨兔肝脏缺血再灌注损伤(IRI)的3.0T MRI表现。方法:将35只新西兰大白兔随机分成假手术组和IRI组(共计7组,每组5只)。建立肝缺血模型的方法为结扎肝左叶血供60 min后恢复血供,分别于恢复血供后0.5、1.5、6.0、12.0、24.0和48.0 h行T1WI、T2WI及增强扫描,并与病理检查结果进行对照分析。结果:在T2WI上,IRI早期(0.5 h、1.5 h)表现整个肝左叶信号增高,随着损伤程度的加重,6 h和12 h肝内出现点片状高信号,且在T1WI增强扫描时呈强化减低区;在24 h和48 h,坏死区在T2WI上呈显著高信号,与未坏死肝组织(无强化区)分界清楚。肝脏缺血再灌注损伤早期镜下表现为肝细胞弥漫性肿胀,肝窦内、汇管区、中央静脉及小动脉内红细胞淤积;随着损伤加重,肝窦肿胀,肝细胞核固缩凋亡,肝窦解离,最后发展为凝固性坏死。结论:3.0T MRI能够动态反映肝脏缺血再灌注损伤的病理发展过程,尤其是缺血再灌注损伤的早期阶段(<2 h),为临床诊断和治疗提供了一种可行性的评价方法。
Objective：To explore the MRI features during the progress of hepatic ischemia reperfusion injury（IRI） in a rabbit model at 3.0T.Methods：Seven groups of New Zealand white rabbits were used（n=5,each）：six IRI groups underwent 60min left lobar ischemia followed by 0.5,1.5,6,12,24 and 48h reperfusion,while the last group was used as the control group.T2-weighted（T2WI）,T1-weighted（T1WI）,and contrast-enhanced T1WI were performed respectively with 3.0T magnetic resonance imaging scanner in each group.Liver samples underwent histological examination after every group had completed the scanning.Results：Compared with the contrast group,the signal of the whole left lobar in 0.5h and 1.5h groups increased obviously on the T2WI and the enhancement of left lobe decreased significantly.With the progression of IRI,T2WI revealed uneven spotty higher signal and showed relatively low enhanced regions at the edge（marginal areas） of the left liver in the 6h and 12h groups.The wedge-shaped and patchy necrotic areas showing high signal without enhancement had marked boundaries from uninfarcted areas on T2WI from 24~48h of reperfusion.Conclusion：3.0T MRI can monitor the pathological process of rabbit liver ischemia reperfusion injury dynamically,especially in the early phase（2h）,and provide a feasible imaging modality for follow-up IRI in clinical diagnosis and treatment.
出处
《放射学实践》
北大核心
2011年第3期271-274,共4页
Radiologic Practice
基金
国家自然科学基金(30800269,30970798)
关键词
磁共振成像
缺血再灌注损伤
肝脏
实验研究
Magnetic resonance imaging
Ischemic reperfusion injury
Liver
Experimental study
