摘要
为建立高效诱导54Q53紫花苜蓿(Medicago sativa)愈伤组织体系,分别研究2种细胞分裂素,3种外植体及4种培养基对愈伤组织诱导的影响,并优化细胞分裂素和生长素的浓度组合。结果表明,采用下胚轴作为外植体在MS基本培养基上添加2.0 mg/L 2,4-D+0.5 mg/L 6-BA为最佳诱导愈伤组织方案。另外,分别在B5、MS基本培养基上添加不同质量浓度及组合的KT、NAA进行愈伤组织再生探讨,在MS基本培养基上添加KT(0.5mg/L)能分化出更多更壮的根,而NAA对出根率有抑制作用。
In order to establish the high efficiency alfalfa （Medicago sativa） regeneration system for callus induction of the 54Q53 lucerne, two different cytokinins, three explants, and four basic solid-media were conducted and concentration combination of the auxin and cytokinin was optimized. The results of this study showed that the best protocol was the hypocotyls as the explants and MS medium with plant hormones, 2.0 mg/L 2,4-D＋0. 5 mg/L 6-BA, as the induction medium. Furthermore, preliminary alfalfa callus regeneration was studied by using the MS or B5 basic medium supplemented with different concentrations and combinations of KT, NAA. More and stronger adventitious roots were obtained on the MS medium containing 0.5 mg/L KT and the root development would be repressed by adding the NAA.
出处
《草业科学》
CAS
CSCD
北大核心
2011年第5期763-768,共6页
Pratacultural Science
基金
山东省农业良种工程牧草项目(2008LZ019-01
2008LZ019-04)
