摘要
目的观察荷负电气溶胶治疗对大鼠烫伤创面愈合过程中白细胞介素（IL）-8、IL-10表达的影响，探讨荷负电气溶胶治疗促进创面愈合的作用机制。方法制作sD大鼠深Ⅱ度烫伤模型，采用分组对照方法，将40只鼠随机分为治疗组（n。=20）和对照组（n2=20）。治疗组应用荷负电气溶胶治疗，每次1．5h，每天2次，直至创面愈合；对照组不作荷负电气溶胶治疗。伤后第1-11天分别取创面标本制作切片，采用免疫组织化学和图像分析方法，检测创面愈合过程中IL-8和IL-10表达水平。结果创面平均愈合时间治疗组为（7．00±1．15）d，对照组为（9．00±1．34）d，治疗组创面愈合时间明显提前（P〈0．01）。免疫组织化学显示，两组IL-8均在伤后第1天开始表达，主要位于多核粒细胞和单核细胞；第3天表达明显增多达高峰，并见大量成纤维细胞表达，治疗组的峰值明显低于对照组，差异有统计学意义（P〈0．01），第5—11天表达水平迅速下降。两组IL-10伤后第1天在淋巴细胞和单核细胞均有表达；第3天开始有角质细胞表达并达高峰，第511天表达水平缓慢下降，但治疗组要明显高于对照组，第3-11天差异有统计学意义（P〈0．01）。结论荷负电气溶胶治疗能有效抑制创面IL-8的表达及促进IL-10的表达，缩短炎症进程，从而加速创面愈合。
Objective To discuss the influence of aerosol bioelectricity on the expression of inter-leukin （IL）-8 and IL-10 in wound healing of burned rats. Methods The deep ]I degree scalding models were established in Sprague Dawley （SD） rats. Rats were randomly divided into experimental group （n2 = 20） and control group （n2 = 20）. The rats in experimental group were treated with aerosol bioelectricity. Samples were collected at the first to eleventh day post-scalding. Immunohistochemistry and image analysis methods were conducted to examine the expression of IL-8 and IL-10 in both experimental and control groups. Results The average wound healing time in experimental group was 7.00 ± 1.15 days, and that in control group was 9. 00 ± 1.34 days. IL-8 and IL-10 were observed mainly in polylmorphonuelear and mononuclear ceils in both experimental and control groups on the 1 st day. On the third day, fibroblasts a-hounded, IL-8 expression was increased evidently and reached a peak. The peak value （6. 73 ± 1. 36 ） in experimental group was lower significantly than that in control group （2. 85 ± 0. 72, P 〈 0. 01 ）. From the 5th to l lth day, IL-8 expression was declined rapidly. IL-10 was expressed in keratode cells and had the peak value in experimental group （ 1.24 ±0. 15） and control group （5.69 ± 1.32） on the 3rd day. IL-10 expression was declined gradually from the 5th to 1 lth days. The expression level of IL-10 in experimental group was significantly higher than in control group from the 3rd day to llth days post-scalding （P〈 0. 01 ）. On the 3rd day, both IL-8 and IL-10 in experimental and control groups were expressed abundantly, and there was negative relationship between them （r = -0. 862,P 〈 0. 0! ）. Conclusion Aerosol bioelectricity can indicate active cells proliferation through down-regulating the expression of IL-8 and up-regulating the expression of IL-10, accelerating burned wound healing.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2011年第5期694-696,共3页
Chinese Journal of Experimental Surgery
基金
广东省自然科学基金资助项目（8451008901000894）
创伤、烧伤与复合伤国家重点实验室开放基金资助项目（SKLKF200808）
