摘要
目的探讨中药免疫调节剂对梗阻性黄疸幼鼠血液和肠黏膜T淋巴细胞免疫功能的影响。方法以3周龄Wistar幼鼠为载体，按随机数字表法随机分为正常对照组、假手术组、梗阻性黄疽（OJ）组、OJ＋中药免疫调节剂组（OJ＋ZY），各12只。除正常对照组外，其余3组结扎胆总管，制作阻塞性黄疸动物模型。利用流式细胞仪检测实验幼鼠外周血及黏膜T淋巴细胞（CD4^＋、CD8^＋）的百分率，计算CD4^＋，CD8^＋比值。结果OJ组外周血、肠黏膜CD4^＋T细胞[分别为（36．2±4．2）％、（28．8±1．8）％]、CD4^＋／CD8^＋比值（分别为1．14±0．39、1．37±0．34），明显低于正常对照组[外周血：CD4^＋（41．5±5．3）％、CD4^＋／CD8^＋（1．37±0．19）；肠黏膜：CD4^＋（32．3±2．4）％、CD4^＋／CD8^＋（1．84±0．28）]及假手术组[外周血：CD4^＋（41．2±5．5）％、CD4^＋／CD8^＋（1．45±0．27）；肠黏膜：cD4’（31．5±2．7）％、CD4^＋／CD8^＋（1．63±0．58）]，差异均有统计学意义（P均〈0．05）。OJ＋ZY组外周血和肠黏膜CD4^＋T细胞（分别为（42．7±6．3）％、（33．6±2．4）％）、CD4^＋／CD8^＋（分别为1．56±0．46、1．84±0．56）均较OJ组升高，两组比较差异有统计学意义（P均〈0．05）。结论中药免疫调节剂可提高OJ幼鼠T淋巴细胞的免疫功能，而正常对照组和假手术组变化不明显。
Objective To evaluate the effect of Chinese herbal medicine immunomodulator on T lymphocyte immune function in peripheral blood and intestinal mucosa of immature rats with obstructive jaundice. Methods Three-weeks Wistar rats were randomly divided into four groups. （n= 12, in each） ： normal control group, sham operation group, obstructive jaundiee（OJ）group, OJ ＋Chinese herbal medicine immunomodulator（OJ＋ZY） group. Except for the normal control group, the others＇ bile duct stones were ligatured to establish rat models with obstructive jaundice. The percentage of CD4^＋ and CD8^＋ tlymphocytes and the ratio of CD4^＋/CD8^＋ in peripheral blood and intestinal mucosa of immature rats was detected by flow cytometry. Results The percentage of CD4^＋cell[（36.2 ± 4.2）%, （28.8± 1.8）% respectively ] and the ratio of CD4^＋/CD8^＋ [ （1.14±0.39）, （1.37±0.34）respectively] in OJ group were lower than those in normal control group [peripheral blood： CD4^＋ （41.5±5.3）%, CD4^＋/CD8^＋ （1.37±0.19） ; intestinal mucosa： CD4^＋（32.3± 2.4） %, CD4^＋/CD8^＋ （1.84±0.28） and sham operation group （peripheral blood： CD4^＋ （41.2 ±5.5） %, CD4^＋/CD8^＋ （1.45±0.27）; intestinal mucosa： CD4^＋（31.5±2.7）%, CD4^＋/CD8^＋ （1.63±0.58）] . The difference was statistical significant （P〈0.05）. The percentage of CD44 cell [ （42.7 ± 6.3） %, （33.6±2.4） % respectively] and the ratio ofCD4^＋/CD8^＋ [ （1.56± 0.46）, （1.84±0.56）respectively] in OJ＋ZY group, were higher than those ill OJ group（P〈0.05）. The difference was statistical significant（P〈0.05）. Conclusion Chinese herbal medicine immunomodulator can increase T lymphocyte immune function in immature rats with obstructive jaundice, but has no significance in normal control group as well as sham operation group.
出处
《国际中医中药杂志》
2011年第5期394-396,共3页
International Journal of Traditional Chinese Medicine
关键词
中药免疫调节剂
梗阻性黄疸
幼鼠
T淋巴细胞免疫功能
Chinese herbal medicine immunomodulator： Obstructive jaundice
Immature rats
Tlymphocyte immune fimction
