摘要
采用水提醇沉法,利用L9(33)正交试验优选锁阳多糖的提取工艺,用硫酸苯酚比色法对甘肃河西沙区不同地区锁阳中多糖含量进行测定。结果表明:提取最佳工艺条件为料液比1:15(g/mL)、提取时间90min、提取次数为2次;甘肃河西沙区不同地区锁阳多糖含量差别显著。锁阳多糖可作为控制锁阳质量的一项指标性成分。
The water extraction and ethanol precipitation of polysaccharides from the roots of Cynomorium songaricum Rupr.were optimized by orthogonal array design and the contents of polysaccharides in samples from different habitats in Hexi Corridor were determined by benzenol-H2SO4 method.Results indicated that the optimal extraction conditions were material/liquid ratio of 1：15（g/mL）,refluxing extraction duration of 90 min and repeated extraction number of 2.Root samples of Cynomorium songaricum Rupr.from different habitats in Hexi Corridor were significantly different from each other in their polysaccharide contents.Therefore,polysaccharide content can be used as a quality control indicator for Cynomorium songaricum Rupr..
出处
《食品科学》
EI
CAS
CSCD
北大核心
2011年第10期79-83,共5页
Food Science
基金
甘肃省科技支撑计划项目(0804NKCG082)
甘肃省教育厅研究生导师计划项目(0809-07)
甘肃省高校河西走廊特色资源利用省级重点实验室项目(XZ0707)
关键词
锁阳
多糖
正交试验
提取工艺
含量测定
Cynomorium songaricum Rupr.
polysaccharide
orthogonal test
extraction process
content determi-nation
