摘要
研究了一种针对禽畜肉中氯霉素残留量的超高效液相色谱-串联质谱联用分析方法.本方法采用乙酸乙酯萃取禽畜肉中的氯霉素,提取液氮吹至干后用超纯水溶解样品残渣,然后用HLB小柱进行固相萃取,经过净化的样品通过超高效液相色谱进行分离,然后用电喷雾质谱负离子多反应监测模式[ESI（-）-MRM]对氯霉素进行检测.该方法的检出限为0.002 5μg/kg,添加水平为0.1~5.0μg/kg时,加标回收率为75.8%~82.5%,相对标准偏差小于10%.该方法具有灵敏度高、选择性好、分析时间短等优点.
A new determination of chloramphenicol in meat was introduced.Solid-phase extraction（SPE） was applied in pretreatment of samples which were separated by ultra performance liquid chromatography（UPLC） with acetronitrile-water as mobile phase afterwards.The detection of chloramphenicol residues was performed by MS/MS in ESI（-）-MRM mode.The results shows that the pretreatment,as well as the analytical approach,was highly efficient,sensitive and also specific to chloramphenicol.
出处
《云南大学学报（自然科学版）》
CAS
CSCD
北大核心
2011年第3期336-339,共4页
Journal of Yunnan University(Natural Sciences Edition)
基金
云南省科技平台项目资助(2009DA022)
