摘要
目的 :探讨血管紧张素转换酶 ( ACE)基因的插入 /缺失 ( insertion/deletion,I/D)多态性与冠状动脉病变的相关性。方法 :应用聚合酶链反应 ( PCR)扩增技术检测 86例行冠状动脉造影患者的 ACE基因 I/D多态性。结果 :冠状动脉异常组的 DD基因型频率 0 .41,D等位基因频率 0 .5 2 ,显著高于冠状动脉正常组的 0 .15和 0 .2 9( P <0 .0 5 ) ;DD基因型与冠状动脉病变有关 ( OR=3.97,P<0 .0 5 )。多支病变与 DD基因型的关系更为密切 ( OR=4.72 ,P<0 .0 5 )。冠状动脉正常组、单支病变组和多支病变组的 DD型频率依次为 0 .15、0 .33和 0 .46( P <0 .0 5 ) ,D等位基因频率为 0 .2 9、0 .44和 0 .5 6( P <0 .0 1)。结论 :ACE基因的 I/D多态性与冠状动脉病变及其严重程度相关 ,DD型及 D等位基因频率随冠状动脉病变及其程度的加重而逐渐升高。在冠状动脉病变患者中 DD及 ID型患者的吸烟率、甘油三酯及血压显著低于 型者 ( P <0 .0 5 ) ,说明 D等位基因及 DD基因型可能是冠心病低危人群冠状动脉病变的重要危险因素之一。
Objective:To probe into the relationship between ACE gene polymorphism and coronary artery lesions in a Chinese population.Method:Polymerase chain reaction (PCR) was used to determine the genotypes for an insertion/deletion polymorphism in intron 16 of the ACE gene in 86 cases which had been performed angiography.Result:The insertion/deletion polymorphism in intron 16 of the ACE gene was determined by PCR and categorized into three genotypes:two deletion alleles (genotype DD),heterozygous alleles (genotype ID),and two insertion alleles (genotype II).The results showed that the frequencies of the genotype patterns of DD,ID,II and the D allele were 15%,27%,58%,29% in 33 cases of normal vessel group and 41%,21%,38%,52% in 53 cases of abnormal coronary artery group.The DD genotype and D allele were more common in abnormal coronary artery patients when compared with normal subjects (41% versus 15%,P< 0.05 and 52% versus 29%,P< 0.05 ,respectively).The DD genotype was associated with coronary artery disease (OR= 3.97 ,P< 0.05 ).Furthermore,the higher the DD genotype and D allele frequencies were,the severer the coronary artery disease was.The DD genotype frequencies were 0.15 , 0.33 , 0.46 (χ 2= 7.406 ,P< 0.05 ) and D allele 0.29 , 0.44 , 0.56 (χ 2= 10.085 ,P< 0.01 ) in normal vessel,single vessel lesion (18 cases) and multi vessel disease (35 cases) respectively.The DD genotype was strongly associated with multi vessel disease (OR= 4.72 ,P< 0.05 ).In addition,although there were no significant difference in age,cholesterol,apo A,apo B,blood sugar among each genotype group,the ratio of smoking,triglyceride and blood pressure (including SBP and DBP) in the II genotype group were significantly higher than that in the ID and DD genotype groups (P< 0.05 ).Conclusion:The deletion polymorphism of the ACE gene is strongly associated with coronary artery disease and the degree of vessel lesion.The genotype DD and D allele seem to be a potent risk factor of coronary artery disease,especially in individuals with low classic risk factors.
出处
《临床心血管病杂志》
CAS
CSCD
北大核心
1999年第10期452-455,共4页
Journal of Clinical Cardiology
基金
广东省医学科研基金!( A1997465 )
关键词
血管紧张素
转换酶
基因多态性
冠状动脉病变
Angiotensin converting enzyme Gene polymorphism Coronary artery disease
