摘要
[目的]构建里氏木霉外源表达载体。[方法]以里氏木霉40359的CBHI启动子和终止子构建了里氏木霉40359外源表达载体,并用该表达载体成功表达了抗潮霉素B磷酸转移酶基因,得到6株可在含175 mg/L潮霉素的基础培养基上生长的抗性菌株;提取6株抗性菌株的DNA整合到里氏木霉菌株中,并对其进行潮霉素耐受性检测。[结果]6株抗性菌株的抗潮霉素能力比原始菌株提高了75%;转基因菌株的潮霉素抗性可以稳定遗传,证明表达载体构建成功。[结论]为开展里氏木霉分子生物学研究与遗传改造奠定了基础。
[Objective] The study was to construct a Trichoderma reesei vector for expressing external genes.[Method] Using CBHI promoter and terminator of T.reesei strain 40359,we constructed an expression vector of T.reesei strain 40359 for expressing Hpt gene and got 6 strains capable of growing on basic medium containing 175 mg/L hygromycin B,further conducted hygromycin resistance test.[Result] In comparison with the original strain（wild type）,hygromycin resistance the 6 engineered strains was increased by 75%;the hygromycin resistance could inherit stably.[Conclusion] Our results laid basis for biological study on T.reesei at molecular and genetically engineering levels.
出处
《安徽农业科学》
CAS
北大核心
2011年第13期7617-7620,共4页
Journal of Anhui Agricultural Sciences
关键词
里氏木霉
载体
遗传转化
Trichoderma reesei
Vector
Genetic transformation
