摘要
目的 探讨可抽提核抗原（ Ｅ Ｎ Ａ） 抗体谱的临床意义及与其他免疫指标的关系。方法 对１５６ 份自身免疫病患者血清样本，采用免疫印迹法检测抗 Ｅ Ｎ Ａ 抗体，经免疫浊度法检测 Ｉｇ 和补体。结果 （１） 抗 Ｅ Ｎ Ａ 抗体在自身免疫病有较高的检出率，其中抗平滑肌（ Ｓｍ） 抗体、抗 Ｕ１ 核糖核蛋白抗体的阳性率在系统性红斑狼疮（ Ｓ Ｌ Ｅ） 中分别为３６ ．３ ％ 、２８ ．８ ％ ； 抗 Ｓ Ｓ Ａ 和抗 Ｓ Ｓ Ｂ 抗体的出现在干燥综合征（ Ｓ Ｓ） 患者中分别为５／１６ 和３／１６ 。（２） 与正常对照和抗体阴性组比较，抗 Ｅ Ｎ Ａ 阳性血清中 Ｉｇ Ｇ、 Ｉｇ Ｍ、 Ｉｇ Ａ 增高，分别为（２１ ．６ ±１５ ．０） 、（１ ．７ ±０ ．８） 、（２ ．２ ±１ ．２）ｇ／ Ｌ， 而 Ｃ３ 、 Ｃ４ 和总补体溶血活性（ Ｃ Ｈ５０）水平下降分别为（１ ．０８ ±０ ．３７） 、（０ ．２１ ±０ ．１２） 、（９８ ．±２９） ｇ／ Ｌ；（３） 随着抗 Ｅ Ｎ Ａ 抗体免疫印迹条带数的增多， Ｉｇ Ｇ 增高，而 Ｉｇ Ｍ、 Ｃ３ 、 Ｃ４ 及 Ｃ Ｈ５０ 与之相反，下降很明显；（４） Ｃ３ 、 Ｃ４ 、 Ｃ Ｈ５０ 与疾病活动性有相关关系。结论 抗 Ｅ Ｎ Ａ 抗体阳性者存在体液免疫应答增加。
Objective To study the clinical significance of anti ENA autoantibody profiles and its relations with other immune markers.Methods Anti ENA autoantibodies were tested with Western blot, while immunoglobulins and complements were detected quantitatively by turbidimetry.Results The high positive rate of anti ENA autoantibody was detected but not specific in several autoimmune diseases. The positive rates of anti Sm and anti U1RNP autoantibodies were 36.3% and 28.8% in SLE respectively. Anti SSA and anti SSB autoantibodies occirred in 31.3% and 18.8% patients with SS. The quantity of IgG, IgM and IgA was increased in anti ENA antibody positive sera, while that of C 3,C 4 and CH 50 was decreased. The elevation of IgG and decrease of IgM, C 3 and CH 50 were correlated with the number of bands of anti ENA autoantibodies by immunoblot. The association between clinical activity of diseases and C 3, C 4 and CH 50 were tested. Conclusion Humoral immune activation was demonstrated in patients with positive NEA autoantibody and positively correlated with the number of bands of anti ENA autoantibodies.
