摘要
AIM:To study the correlation between high metastasisassociated protein 1(MTA1)expression and lymphangiogenesis in colorectal cancer(CRC)and its role in production of vascular endothelial growth factor-C(VEGF-C). METHODS:Impact of high MTA1 and VEGF-C expression levels on disease progression and lymphovasculardensity(LVD,D2-40-immunolabeled)in 81 cases of human CRC was evaluated by immunohistochemistry. VEGF-C mRNA and protein expressions in human LoVo and HCT116 cell lines were detected by real-time polymerase chain reaction and Western blotting,respectively,with a stable expression vector or siRNA. RESULTS:The elevated MTA1 and VEGF-C expression levels were correlated with lymph node metastasis and Dukes stages(P<0.05).Additionally,high MTA1 expression level was correlated with a large tumor size(P< 0.05).A significant correlation was found between MTA1 and VEGF-C protein expressions in tumor cells(r=0.371, P<0.05).Similar to the VEGF-C expression level,high MTA1 expression level was correlated with high LVD in CRC(P<0.05).Furthermore,over-expression of MTA1 significantly enhanced the VEGF-C mRNA and protein expression levels,whereas siRNAs-knocked down MTA1 decreased the VEGF-C expression level. CONCLUSION:MTA1,as a regulator of tumor-associated lymphangiogenesis,promotes lymphangiogenesis in CRC by mediating the VEGF-C expression.
AIM：To study the correlation between high metastasisassociated protein 1（MTA1）expression and lymphangiogenesis in colorectal cancer（CRC）and its role in production of vascular endothelial growth factor-C（VEGF-C）. METHODS：Impact of high MTA1 and VEGF-C expression levels on disease progression and lymphovasculardensity（LVD,D2-40-immunolabeled）in 81 cases of human CRC was evaluated by immunohistochemistry. VEGF-C mRNA and protein expressions in human LoVo and HCT116 cell lines were detected by real-time polymerase chain reaction and Western blotting,respectively,with a stable expression vector or siRNA. RESULTS：The elevated MTA1 and VEGF-C expression levels were correlated with lymph node metastasis and Dukes stages（P0.05）.Additionally,high MTA1 expression level was correlated with a large tumor size（P 0.05）.A significant correlation was found between MTA1 and VEGF-C protein expressions in tumor cells（r=0.371, P0.05）.Similar to the VEGF-C expression level,high MTA1 expression level was correlated with high LVD in CRC（P0.05）.Furthermore,over-expression of MTA1 significantly enhanced the VEGF-C mRNA and protein expression levels,whereas siRNAs-knocked down MTA1 decreased the VEGF-C expression level. CONCLUSION：MTA1,as a regulator of tumor-associated lymphangiogenesis,promotes lymphangiogenesis in CRC by mediating the VEGF-C expression.
基金
Supported by National Natural Science Foundation of China(No.30772131)
Fundamental Research Funds for Central Universities(No.21609313)from Ministry of Education of China
