摘要
目的探讨燕麦葡聚糖对高胆固醇血症大鼠血管内皮活性物质及炎症反应标志物C-反应蛋白(CRP)的影响,为防治动脉粥样硬化提供理论依据。方法雄性SD大鼠44只随机分为正常对照组(灌饲生理盐水)11只,高胆固醇模型组(以下简称模型组)33只。模型组给予高脂饲料,正常对照组给予基础饲料,连续饲养4周后测定大鼠空腹血清总胆固醇(TC),以模型组血清TC水平显著高于正常对照组为确定造模成功的指标。将高胆固醇血症大鼠随机分为3组,高胆固醇对照组、葡聚糖高低剂量组[剂量分别为1 000和500 mg/(kg.d)],两个对照组灌胃生理盐水,实验结束,取血测定一氧化氮(NO)、内皮素(ET-1)、血栓素(TXA2)和CRP含量。结果与高胆固醇对照组比较,燕麦葡聚糖能够升高NO水平,降低ET-1、TXA2含量,且一定剂量的燕麦葡聚糖可以降低CRP水平。结论燕麦葡聚糖具有一定的改善血管内皮舒缩功能,抑制血小板聚集,减轻内皮炎症反应,具有保护血管内皮的作用。
Objective To observe the effects of oats glucan on vascular endothelial active substances and inflammatory markers（CRP） in hypercholesterolemic rats.MethodsMale SD rats were randomly divided into normal group（11 rats） administered with distilled water and high cholesterol group（33 rats） fed with high fat diet for 4 weeks.It was applied as an indicator for the success of inducing hypercholesterolemic model when the levels of fasting serum total cholesterol（TC） in the high cholesterol group was significantly higher than the control group.The hypercholesterolemic rats were regarded as the model group and then divided into three subgroups,high cholesterol control group and two oat glucan groups fed with different doses of oat glucan [500 and 1000 mg/（kg·d）].The rats in control groups were given water,and the rats in oar glucan groups were fed with oat glucan for another 6 weeks.Vascular endothelial function indexes,including nitric oxide（NO）,endothelium（ET-1）,thromboxane A2（TXA2）,and inflammatory marker C-reactive protein（CRP） were tested.Results The levels of NO were increased and the levels of ET-1and TXA2 were decreased in the oat glucan groups compared with the high cholesterol control group,and the levels of CRP was decreased with higher dose of oat glucan.ConclusionThere might have some effects of oat glucan on improving the vascular endothelial function,inhibiting platelet aggregation,reducing inflammatory response and protecting vascular endothelial cells.
出处
《中国食品卫生杂志》
北大核心
2011年第3期233-235,共3页
Chinese Journal of Food Hygiene
关键词
燕麦葡聚糖
高胆固醇血症
内皮功能
保护作用
炎症反应
Oat glucan
hypercholesterolemia
endothelial function
protective effect
inflammatory response
