摘要
目的通过动物实验验证载银硅灰石涂层体内抗菌性能。方法将22只新西兰大白兔随机分为两组,随机取一侧股骨髁,试验组植入载银硅灰石钛板,对照组植入普通硅灰石钛板。术后第12天于术侧膝关节腔内注射金黄色葡萄球菌（ATCC25923）菌液10-8CFU。两组实验钛板的抗菌性能经实验兔肛温、体重变化,X线摄片结果,钛板、螺钉、骨组织、周围软组织以及组织液细菌培养结果,局部骨组织病理切片观察结果进行表征。两组实验钛板的表面特性经扫描电镜观察进行分析。结果两组实验兔在第12天注射金黄色葡萄球菌后体温均上升,试验组金黄色葡萄球菌阳性率显著低于对照组,细菌培养阳性的钛板表面可见明显细菌生物膜附着,部分病理切片样本革兰染色阳性,与其细菌培养结果相符。结论载银硅灰石涂层与普通硅灰石涂层相比,具有显著的体内抗菌性能。
Objective To investigate the antibacterial properties of plasma sprayed wollastonite/silver coatings in vivo by animal experiment.Methods Randomly,22 rabbits were divided into two groups.The titanium plates with wollastonite/silver coatings were implanted into one femoral condyle of the rabbit in the experimental group randomly while the titanium plates with wollastonite coatings in the control group.The Staphylococcus aureus serum（ATCC25923）10~8 colony-forming units was injected into the operated knee lumen on the 12th day.The antibacterial activity against Staphylococcus aureus was examined by each rabbit＇s clinical signs（anus temperature and weight）,radiography,bacteria cultivation of the titanium plates,screws,bone tissue,soft tissue and tissue fluid around,as well as the pathological section of local bone tissue.The surface characteristics of wollastonite/silver and wollastonite coating were investigated by scanning electron microscopy.Results Temperatures of rabbits in two groups rose after injection of Staphylococcus aureus serum on the 12th day.The bacteria positive rate of the experimental group was significantly lower than that of the control group.The bacterial biofilm could be obviously observed on the surface of the infected plates.The Gram＇s staining of some pathological section was positive,which was consistent with the result of the bacterial cultivation.Conclusion Compared with the wollastonite coatings,the antibacterial properties of the wollastonite/silver coatings in vivo is more valuable.
出处
《国际骨科学杂志》
2011年第3期185-188,共4页
International Journal of Orthopaedics
基金
上海市科委基础研究重点项目(07JC14057)
上海市科委纳米科技专项基金(0852nm03300)
国家自然科学基金(30700170)
关键词
兔动物模型
等离子喷涂
钛
银
抗菌性能
Rabbit animal model Plasma spraying Titanium Silver Antibacterial properties
