摘要
目的:观察黄芪多糖(Astragalus Polysaccharides,APS)对2型糖尿病(T2DM)患者内皮祖细胞(EPCs)体外增殖的影响。方法:密度梯度离心法获取T2DM患者外周血单个核细胞,在鼠尾胶原包被的培养瓶中培养7 d后鉴定EPCs,MTT检测APS对T2DM患者EPCs增殖的影响。结果:T2DM外周血单个核细胞在鼠尾胶原包被的培养瓶中培养后呈梭形、铺路石样,表达内皮细胞的特异性抗原CD34和KDR,表达干/祖细胞抗原CD133;APS在一定剂量和时间范围内能促进T2DM外周血EPCs增殖(P<0.05)。结论:鼠尾胶原可代替EPCs培养中常用的纤维连接蛋白,是体外分离培养外周血EPCs的更节省的一种方法,APS能促进EPCs增殖。
Objective： To investigate the methods of isolating and culturing endothelial progenitor cells（EPCs） from peripheral blood in patients with type 2 diabetes（T2DM）,and to observe the effect of astragalus polysaccharides（APS） on proliferation of EPCs.Methods： Total mononuclear cells were isolated with density gradient centrifugation and put into the culture flasks previously coated by rat tail collagen.After 7 days of culture,EPCs were identifyed,and treated with various concentrations of APS for different durations.Proliferation of EPCs was measured by MTT.Results： Most of the cultured cells adhering to the culture flask displayed fibroblast-like and slabstone-like morphology.Endothelial cell specific antigen CD34 and KDR,and progenitor antigen CD133 were expressed.APS could promote the proliferation of EPCs in T2DM in certain ranges of dose and time（P0.05）.Conclusions： The rat tail collagen can be a cheaper replacement of fibronectin for the culture of EPCs from peripheral blood in T2DM.APS could markedly promote the proliferation of EPCs in T2DM.
出处
《贵阳医学院学报》
CAS
2011年第2期119-123,共5页
Journal of Guiyang Medical College
基金
国家自然科学基金资助课题(30960491)
关键词
内皮
血管
细胞培养
黄芪多糖
糖尿病
非胰岛素依赖型
endothelium
vascular
cell culture
astragalin polysaccharides
diabetes mellitus
non-insulin dependent
