摘要
采用Biolog方法、最大或然数和主成分分析方法研究了某采油区污染场地及周围土壤的污染特征、微生物数量、微生物群落功能多样性以及相互作用关系。结果表明,土壤均一定程度受到石油污染,污染场地土壤呈现以萜类、姥鲛烷及植烷等难降解物质为主的污染特征,其土壤中微生物数量最多,总活菌数为1.73×1010个/g,石油烃降解菌数为7.50×106个/g;石油污染对微生物群落结构与功能多样性影响较大,污染严重土壤微生物群落代谢活性较强、群落结构丰富、种群数量及常见物种较多、均一性较好;石油污染土壤微生物群落结构存在一定相似性,根据主成分的变量系数得出,污染严重土壤微生物群落倾向利用羧酸和碳水化合物。
4 soil samples were collected from oil contaminated site and surrounding site to study the contamination features,microbe quantity,community functional diversity and their interaction relations,methods such as most probable number,Biolog and principal component analysis were adopted in the investigation.The results showed that all sampling soils were contaminated to some extent,and the pollution of site soil was particularly serious.The mainly contained refractory organics in site soil were terpenes,pristane and phytane.The quantity of microbe in site soil was also the most with the total viable number of bacteria was 1.73×1010 per gram,hydrocarbon degrading bacteria was 7.50×106 per gram.Petroleum hydrocarbon had great impact on microbial community structure and functional diversity,as for the most contaminated soil,the microbial community metabolic activity was relatively strong,community structure was abundant,it had more populations and common species with better homogeneity.There were a certain similarity on microbial community structure for oil contaminated soils,which microbes tended to metabolize carboxylic acids and carbohydrates.
出处
《环境污染与防治》
CAS
CSCD
北大核心
2011年第5期54-58,共5页
Environmental Pollution & Control
基金
国家"863计划"项目(No.2007AA06Z343)
国家自然科学基金资助项目(No.200950879029)
关键词
石油烃
降解菌
Biolog方法
群落多样性
petroleum hydrocarbon
degrading bacteria
Biolog method
community diversity
