摘要
研究枯草芽孢杆菌(Bacillus subtilis)fmbJ产生的Fengycin对桃软腐病菌(Rhizopus stolonifer)呼吸代谢、线粒体NADH脱氢酶活性、琥珀酸脱氢酶活性及营养物质利用的影响。通过比较典型抑制剂(磷酸钠、碘乙酸和丙二酸)对Fengycin的叠加率。结果表明,Fengycin主要抑制Rhizopus stolonifer呼吸代谢的HMP途径。酶活性实验结果表明:在体内作用时,随着Fengycin浓度的增大,线粒体NADH脱氢酶活性逐渐减小,而体外活性变化不大;随着Fengycin浓度的增大,体内和体外作用都使琥珀酸脱氢酶活性呈现先降低后升高的现象。此外,Fengycin还使Rhizopus stolonifer对糖和蛋白质的利用受到抑制,蛋白合成受到影响。结论:Fengycin作用后,使Rhizopusstolonifer呼吸代谢受阻,合成和分解代谢的平衡被打破,引起病变,生长受到抑制。
The effect of fengycin produced by Bacillus subtilis fmbJ on the respiration,mitochondrial NADH dehydrogenase activity,succinic dehydrogenase and nutrients utilization of Rhizopus stolonifer was studied in this paper.The results showed that fengycin mainly restrained the hexose monophosphate pathway.With the increase of fengycin concentration,the activity of NADH dehydrogenase was decreased in vivo,while kept stable in vitro.Both in vivo and in vitro,the activity of succinic dehydrogenase stepped down first and then went up with fengycin concentration.Furthermore,fengycin had an important impact on the utilization of carbohydrates and protein as well as protein synthesis in R.stolonifer.Therefore,fengycin could inhibit the respiratory metabolism and break the balance between anabolism and catabolism in R.stolonifer,subsequently inducing the pathological changes and growth inhibition.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2011年第11期248-254,共7页
Food Science
基金
国家自然科学基金项目(3067146030871753)
江苏省高新技术计划项目(BG2007337)
